Degree | Type | Year |
---|---|---|
4313802 Advanced Genetics | OT | 0 |
You can view this information at the end of this document.
There are not specific requirement for this module. A certain background on human genetics and DNA damage response, including DNA repair mechanism, is advisable.
The aim of this module is to dissect the genetic basis of cancer predisposition. We will discuss and perform laboratory experiments to explore the molecular basis of syndromes such as hereditary breast cancer, familiar colon cancer or rare DNA repair syndromes characterized by cancer predisposition such as Xeroderma pigmentosum, Fanconi anemia or Lynch syndromes. We will also discuss and experimentally check how DNA repair can be targeted for specific anticancer therapies.
Theoretical lectures and seminars:
-Molecular basis of cancer predisposition: DNA repair and DNA damange response mechanisms.
-BRCAness and genetic predisposition to breast/ovarian cancer
-Rare DNA repair syndromes of cancer predisposition
-Targeting DNA repair in cancer treatment: chemosensitazing tumors by inhibiting DNA repair.
Synthetic lethality: Chemotherapy of BRCA tumours with PARP inhibitors
Laboratory experiments:
-Sensitivity of cells from Xeroderma pigmentosum to UV light
-Repair kinetics of UV-light induced pyrimidine dimers
-Sensitivity of BRCA negative cells to PARP inhibitors
-Homologus recombination assay
These experiments will require the following techniques:
-culturing of human cells from cancer predisposition syndromes
-cytotoxicity and cell cycle assays
-subnuclear local irradiation of fibroblast
-Plasmid transfection
-RNA interference by siRNA
-flow cytometry
-Western blot
-flow cytometry
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Lectures | 10 | 0.4 | |
Seminars | 3 | 0.12 | |
Type: Supervised | |||
Discusion of results and preparation of PPT files | 20 | 0.8 | |
Laboratory experiments | 40 | 1.6 | |
Laboratory report | 30 | 1.2 | |
Type: Autonomous | |||
Studing and reading | 37 | 1.48 |
The student will receive several lectures to get the theoretical background required to perform and follow the laboratory experiments that will take most of the time in close contact with specialists in the field of this module. Bibliografy will be given in advance via Campus Virtual so that the students can discuss it, summaryze it or prepare PPT files in the format of seminars. The students will have to summarize the laboraty experiments performed in the laboratory.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Exam | 40 | 2 | 0.08 | 1, 2, 4, 5, 7, 8, 9, 10 |
Participation | 20 | 2 | 0.08 | 12 |
Report | 40 | 6 | 0.24 | 3, 12, 11, 6, 13, 10 |
Stdents must pass a final exam to demonstrate that they have reach an acceptable degree of acknowledge on the different topics discussed in the module.
Students will have to write a report of the experiments performed which will be corrected and evaluated.
Active participation in the laboratory and via Campus Virtual will be another source of information to reach the final qualification.
The student will receive a list of references and the corresponding PDF documents via Campus Virtual
No applicable
Name | Group | Language | Semester | Turn |
---|---|---|---|---|
(PLABm) Practical laboratories (master) | 1 | English | first semester | morning-mixed |
(TEm) Theory (master) | 1 | English | first semester | morning-mixed |