Introduction to Microbiology, Immunology and Cell Cultures
Code: 106826 ECTS Credits: 6| Degree | Type | Year |
|---|---|---|
| 2504602 Nanoscience and Nanotechnology | OB | 2 |
Contact
- Name:
- Maria del Carme Nogues Sanmiquel
- Email:
- carme.nogues@uab.cat
Teachers
- José Ramón Palacio Cornide
- Jose Luis Corchero Nieto
Teaching groups languages
You can view this information at the end of this document.
Prerequisites
This subject does not need any requirements.
Objectives and Contextualisation
The subject Introduction to Microbiology, Immunology and Cell Culture, is taught in the 2nd semester of the 2nd year of the degree of Nanoscience and Nanotechnology in the Faculty of Sciences. This is a subject with a certain degree of specialization that is divided into three large blocks (Microbiology, Immunology and Cellular cultures) in which the students is expected to acquire some basic notions to begin with the methodologies used in the culture and manipulation of bacterial cells, in immunology laboratories and in cultures and manipulation of eukaryotic cells. That is why it is a subject with an important practical component.
Objectives of the subject:
1) To know the bacterial cell
2) To know the basic methodologies used in a Microbiology laboratory
3) To know the basic concepts of Immunology
4) To know the basic methodologies used in a Immunology laboratory
5) To know the basic equipment of a Cell Culture Laboratory
6) To know the basic methodologies used in a Cell Culture Laboratory
Learning Outcomes
- CM22 (Competence) Identify innovations in nanobiotechnology and their economic and social impact on the field of health.
- CM22 (Competence) Identify innovations in nanobiotechnology and their economic and social impact on the field of health.
- KM37 (Knowledge) Describe fundamental techniques in cell culture, the biology of microorganisms and the immune system.
- KM37 (Knowledge) Describe fundamental techniques in cell culture, the biology of microorganisms and the immune system.
- SM32 (Skill) Use digital tools and documentary sources to obtain, analyse and present information from a critical perspective in the field of nano biotechnology, both orally and in writing.
- SM32 (Skill) Use digital tools and documentary sources to obtain, analyse and present information from a critical perspective in the field of nano biotechnology, both orally and in writing.
- SM32 (Skill) Use digital tools and documentary sources to obtain, analyse and present information from a critical perspective in the field of nano biotechnology, both orally and in writing.
- SM33 (Skill) Use the basic methodologies used in microbiology, immunology, cell culture and molecular biology.
- SM33 (Skill) Use the basic methodologies used in microbiology, immunology, cell culture and molecular biology.
Content
Theory program
Microbiology
- Introduction to Microbiology
- Levels of organization
- The bacterial cell
- Techniques for observing microorganisms
- Isolation and culture techniques of microorganisms
- Techniques for sterilization and preservation of microorganisms
Immunology
- Basic principles of immunology: innate immunity and acquired immunity. The immune system: anatomy, cells, and molecules.
- Components of innate immunity. Mechanisms of innate immunity. Connection between innate and acquired immunity.
- Components of acquired immunity. Mechanisms of acquired immunity.
- Immune response to pathogens
Cell Cultures
- Introduction to cell cultures
- Types of cell cultures
- Physical and biological conditions for cell cultures
- Cell characterization techniques
- Biocompatibility study techniques
Practical Program
Microbiology
- Microorganism counting
- Methods for isolating microorganisms
- Microorganism observation
- Microorganism identification
- Ubiquity and microbial diversity
Immunology
- Separation of peripheral blood leukocytes
- Cell counting using a Neubauer chamber and vital staining
- Determination of cell concentration and viability in the sample
Cell Cultures
- Culturing a cell line
- Freezing/thawing a cell line
- Induction and detection of apoptosis in a cell line
- Detection of actin filaments. Fluorescence microscopy observation.
- Confocal laser scanning microscopa observation of cells incubated with nanoparticles.
Activities and Methodology
| Title | Hours | ECTS | Learning Outcomes |
|---|---|---|---|
| Type: Directed | |||
| Classes teoricas | 31 | 1.24 | CM22, KM37 |
| Laboratory classes | 27 | 1.08 | SM32, SM33 |
| Type: Supervised | |||
| Personalized tutorials | 4 | 0.16 | CM22, KM37, SM32 |
| Type: Autonomous | |||
| Individual study | 77 | 3.08 | CM22, KM37 |
| Preparation of the laboratory report | 4.5 | 0.18 | SM32, SM33 |
The subject of Microbiology, Immunology, and Cell Cultures consists of theoretical master classes and practical laboratory sessions.
The theoretical master classes (31 h) will be conducted using audiovisual material prepared by the professor, which students will have access to on the Virtual Campus (CV) of the UAB before the sessions.
The practical classes are designed to help students learn how to use laboratory instruments and complement their theoretical training. Students will participate in a total of 9 practical sessions, totalling 27 h. They will work in groups of 2. In some sessions, they will need to fill out a sheet with the results. At the end or during the practical sessions, the results from different groups will be shared and discussed collectively.
In the case of the cell culture module, students will need to submit a PowerPoint presentation. In this assignment, they should present the results and discuss whether they meet the expected outcomes, providing reasoning.
Note: 15 minutes of a class, as per the schedule established by the institution/program, will be reserved for students to complete evaluations of the teaching performance and the subject/module
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Assessment
Continous Assessment Activities
| Title | Weighting | Hours | ECTS | Learning Outcomes |
|---|---|---|---|---|
| Delivery of practical laboratory results | 5 | 1.5 | 0.06 | SM32, SM33 |
| Laboratory exam | 20 | 1 | 0.04 | SM33 |
| Theory exam | 75 | 4 | 0.16 | CM22, KM37 |
To pass the subject, you will need to obtain a minimum overall score of 5 out of a maximum of 10 possible points. The scheduled assessment activities are as follows:
Theory:
Represents 75% of the final grade for the subject and is evaluated through two exams.
First theory exam: Represents 37.5% of the final grade and covers approximately half of the material taught in theoretical classes. A score ≥4.5 in the first theoretical exam allows averaging with the second theory exam. Scores ≤4.49 require taking the recovery exam for this part of theory.
Second theory exam: Represents 37.5% of the final grade and covers approximately half of the material taught in theoretical classes. A score ≥4.5 in the second theoretical exam allows averaging with the first theory exam. Scores ≤4.49 require taking the recovery exam for this part of theory.
Practicals:
Represent 25% of the final grade and are assessed through a single exam and the presentation of results obtained during practical sessions. Attendance in laboratory practicals is mandatory. There are a total of 9 sessions. Non-attendance in one, two, or three sessions reduces the practical grade by 20%, 50%, and 80%, respectively. Non-attendance in 4 or more practical sessions results in a “Not Present” for this block.
Exam on techniques used and results obtained in the laboratory: Represents 20% of the final grade. A score ≥4.5 in the practical exam allows averaging with the second theory exam. Scores ≤4.49 require taking the recovery exam for this part.
Presentation of results obtainedduring practicals: Represents 5% of the final grade. Practical work is done in pairs. Each pair must submit a report on their own results, comparing them with the expected outcomes for the group. The submission deadline is ten days after the completion of practical sessions through the Virtual Campus (CV).
Subject grade calculation: Theory exam (37.5% + 37.5%) + Practical exam (20%) + Practical work (5%)
Recovery: To participate in the recovery, students must have been previously evaluated in a set of activities whose weight corresponds to at least two-thirds of the total grade for the subject or module. Therefore, students will receive the qualification of “Not Evaluable” when the assessment activities performed have a weight lower than 67% in the final grade. There will be a recovery exam for students who have not obtained a score higher than 4.49 in any of the three exams (two theory exams and one practical exam), and for students who, after averaging with other subject grades, do not achieve a score equal to or higher than 5. Students only need to retake the exam they did not pass
Bibliography
Relevant Bibliography
Microbiology:
*Madigan, MT, JM Martinko, PV Dunlap, DP Clark. 2015. Brock Biology of Microorganisms. 14ª ed. Pearson Educación, S.A. (en paper i electrònic)
*Willey, J, LM Sherwood, CJ Woolverton. 2009. Microbiología de Prescott, Harley y Klein. 7ª ed. MacGraw-Hill-Interamericana de España. ISBN: 978-84-481-6827-8.(en paper i electrònic)
*Jennifer Louten. 2016. Essential human virology. Elsevier Ed. ISBN: 978-0-12-800947-5
*Microbiología: conceptos esenciales. Jordi Barbé García [i 39 més]. Editorial Médica Panamericana | 2019
Immunology:
*Inmunología J.Kuby, J.A. Owen, J. Punt, S.A. Strandord 7ma Ed 2014 (en paper i electrònic)
*L. Fainboim, J. Geffner. Introducción a la Inmunología Humana. 6ª edición, 2011, Editorial Panamericana. ISBN:978-9500602709 (en paper i electrònic)
*J. R. Regueiro, C. López Larrea, S. González Rodríguez, E. Martínez Naves. Inmunología: Biología y patología del sistema inmunitario, 4ª edición, 2010, Editorial Panamericana. ISBN: 978-8498350036
Cell Cultures
* A. Doyle and J.B. Griffiths Eds. Cell and Tissue Culture: Laboratory procedures in biotechnology. John Wiley & Sons Ltd. 1999. ISBN: 978-0471982555 (no hi ha cap nova edició)
* R.I. Freshney. Culture of Animal Cells: A manual of basic technique. 7th Ed. Wiley-Liss, Inc. 2010. (biblioteca 6e ed. en paper i electrònic). ISBN: 978-1-118-87365-6
* J.P. Mather and D. Barnes Eds. Animal Cell Culture Methods. Methods in Cell Biology. Academic Press. 1998.(en paper i electrònic). ISBN: 978-0124800403
Software
No software will be used
Language list
| Name | Group | Language | Semester | Turn |
|---|---|---|---|---|
| (PLAB) Practical laboratories | 1 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 2 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 3 | Catalan/Spanish | second semester | morning-mixed |
| (TE) Theory | 1 | Catalan/Spanish | second semester | morning-mixed |