Integrated Laboratory Class 2
Code: 100927 ECTS Credits: 3| Degree | Type | Year |
|---|---|---|
| 2500253 Biotechnology | OB | 1 |
Contact
- Name:
- Benet Gunse Forcadell
- Email:
- benet.gunse@uab.cat
Teaching groups languages
You can view this information at the end of this document.
Prerequisites
It is recommended that students are simultaneously taking or have already taken the theory courses corresponding to the contents of the laboratory practices.
You must have passed the safety test in laboratories. The test is answered in the corresponding space of the Virtual Campus and the information that must be consulted is in the space of communication of the Degree in Biotechnology.
Objectives and Contextualisation
The Integrated Laboratory 2 is the second subject of a group of 6 that are distributed over the 6 semesters corresponding to the first three years of the Degree in Biotechnology.
The training objectives of these subjects focus on the acquisition of competences within the framework of the practical training of the student.
The Integrated Laboratory 2 has as its training objectives the acquisition of practical skills in 4 specific contents:
- Organic Chemistry.
- Genetics.
- Animal and Plant Biology.
- Plant Physiology.
The specific objectives of each of the parts of the subject are the following:
Organic Chemistry: With the first two practices, the student is expected to acquire techniques such as the manipulation of an pH meter and the assessments. With the remaining four practices it is intended that students know the basic techniques of work in an organic chemistry laboratory, as well as reflux, distillation, filtration, extraction and some techniques to determine the purity of the obtained products (melting and boiling points , Chromatography in thin layer). You will also learn to search for information in books such as the Handbook of Chemistry and the Merck Index.
During the realization of the practices the students will have to apply the knowledge assimilated to the subject of organic chemistry related to the reactivity of the organic molecules.
Genetics: Recognize the morphology of Drosophila and learn about the life cycle and cytogenetics of this organism in order to manipulate it at the genetic level. Experimenting the concepts of Mendelian genetics (principles of segregation, genes and alleles, relationship of dominance, type of inheritance, concepts of genotype and phenotype, recombination) in the development of a genetic map of three markers, using Drosophila As a model. Use different cytogenetic techniques for the preparation and observation of chromosomes. Identifynormal karyotypes and mutant kariotypes, and learn how to relate them to a certain phenotype. Apply computer tools for the estimation and interpretation of the genetic variation of blood groups in human populations.
Animal and Plant Biology: Correct use of optical material for the observation of fauna and flora (binocular magnifying glass, microscope). Recognize the anatomical and morphological characteristics of the different animal groups. Identify and locate taxonomically the observed animal species.
Learn to recognize fungi, algae, cyanobacteria, aquatic photosynthetic eukaryotes and significant plants in the world of biotechnology through its morphological characteristics.
Plant Physiology: Describe the molecular, cellular and physiological bases of the organization, functioning and integration of living organisms within the framework of their application to biotechnological processes.
Learning Outcomes
- CM24 (Competence) Review the general safety standards of a biotechnology laboratory.
- KM22 (Knowledge) Relate the physiology of different organs and the various metabolic states of an organism.
- KM23 (Knowledge) Recognise the key microscopic characteristics that distinguish prokaryotic cells from eukaryotic cells, and animal cells from plant cells.
- SM21 (Skill) Use prokaryotic and eukaryotic cell culture techniques and techniques for the manipulation of biological systems.
- SM21 (Skill) Use prokaryotic and eukaryotic cell culture techniques and techniques for the manipulation of biological systems.
- SM22 (Skill) Use analytical methodologies for the assay of biological activity of cellular components.
- SM22 (Skill) Use analytical methodologies for the assay of biological activity of cellular components.
Content
Organic chemistry:
Measurement of the pH, relative strength of acids and bases: use of pHmeter and preparation of buffer solutions.
Determination of the molecular mass of an acid: evaluate a dissolution with an indicator
Reduction of one ketone in alcohol: obtaining benzhydrol from benzophenone. Applied techniques: crystallization, suction filtration, determination of the melting point, thin layer chromatography
Sterilification of an alcohol: preparation of isoamyl acetate from isoxylic alcohol. Applied techniques: reflux, extraction, distillation at atmospheric pressure and determination of the purity according to the boiling point
Genetics:
Introduction to the biology and morphology of Drosophila.
Preparation of a three-marker genetic map.
Observation of chromosomes and mutations.
Genetic variability of blood groups in human populations.
Animal Biology:
Protestoms ecdisozous. Nemathodes. Basic characters Adaptations to different types of life. Biological cycles but representative. Biotechnological use of entomopathogenic nematodes.
Hexapods (Insects). Main groups Insects and man.
Deuterostoms. Echinoderms. General organization of the group and adaptive diversification.
Plant Biology:
Fungi: Basic characters, morphology and structures. Modifications and biotechnological use.
Cyanobacteria and aquatic photosynthetic eukaryotes. Basic characters, morphology and structures. Modifications and biotechnological exploitation.
Viridiplantae. Basic characters, morphology and structures. Modifications and biotechnological exploitation.
Plant Physiology:
Water relations: Water potential and plasmolysis
Determination of the physiological effects of a plant hormone (Citokinins)
Study of photosynthesis.
Study of transpiration.
Activities and Methodology
| Title | Hours | ECTS | Learning Outcomes |
|---|---|---|---|
| Type: Directed | |||
| Animal Biology | 6 | 0.24 | CM24, KM22, KM23, SM21, SM22 |
| Genetics | 12 | 0.48 | CM24, KM22, KM23, SM21, SM22 |
| Organic Chemistry | 18 | 0.72 | CM24, KM22, KM23, SM21, SM22 |
| Plant Biology | 6 | 0.24 | CM24, KM22, KM23, SM21, SM22 |
| Plant Physiology | 12 | 0.48 | CM24, KM22, KM23, SM21, SM22 |
| Type: Autonomous | |||
| Autonomous work | 14 | 0.56 | CM24, KM22, KM23, SM21, SM22 |
In general, all the activity in the laboratory is at the same time guided, when the teacher imparts the relevant explanations and supervised when the students carry out their work aunomously.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Assessment
Continous Assessment Activities
| Title | Weighting | Hours | ECTS | Learning Outcomes |
|---|---|---|---|---|
| Animal Biology | 6/54 | 1 | 0.04 | CM24, KM22, KM23, SM21, SM22 |
| Genetics | 12/54 | 2 | 0.08 | CM24, KM22, KM23, SM21, SM22 |
| Organic Chemistry | 18/54 | 2 | 0.08 | CM24, KM22, KM23, SM21, SM22 |
| Plant Biology | 6/54 | 1 | 0.04 | CM24, KM22, KM23, SM21, SM22 |
| Plant Physiology | 12/54 | 1 | 0.04 | CM24, KM22, KM23, SM21, SM22 |
Since this is a practical subject, according to article 112 ter. of the modification of the Academic Regulation RD 1293/2007, this subject does not contemplate systems of recovery.
Single assessment: The students who are accepted will take a synthesis test on a date to be agreed with the head of the subject and this test will have a weight corresponding to 40%, while the rest (practice reports, attendance, attitude ...) will be evaluated as in the continuous evaluation, with the percentages that correspond to each module.
Organic chemistry: The assessment will consist of a part of continuous evaluation (work in the laboratory, laboratory book, questions to be answered) as well as a final exam that could be scheduled the same day as the afternoon practices end, or the Next week The continuous evaluation will have a weight of 40%, and 60% the mark of the practice exam.
Genetics: Each of the practices will be evaluated with a written test of 5 single answer questions with three options to choose from. In the end, the notes of the four laboratories will be counted and a single note will be taken. Practice 4 will be evaluated in situ.
Animal Biology: At the end of each practice the student will have to answer a questionnaire to evaluate that he has obtained the knowledge and the specific competences of each practice
Plant Biology: An examination will be made up of two parts: Visum and Description of a fruit / fruit or modification of the corm where the achievement of the basic objectives of the module will be evaluated.
Plant Physiology:A final individual written test will be done on the last day of the practical course and it will account for 80% of the mark. The practice notebook will be carried out in groups and will account the remaining 20% of the mark. The notebook will be delivered via Virtual Campus one week after the end of the practical course.
Overall course grade: It will be possible to compensate between modules if any of them is suspended with a note greater than 4.5 and if the calculation of the weighted average is superior to 5, understanding that the student has had to submit to 100% of the evaluation tests. In the event that a module does not reach this minimum mark of possible compensation or the student has not been presented to any of the parties, the subject will be suspended with a 4, regardless of whether the calculation exceeds 5. In If the weighted note is less than 4, the overall mark will be the result of the calculation obtained. In the event that the subject is suspended, the marks over 5 of the surpassed parts will remain in the face of the next course, so that the student does not have to be presented again to these parts, although if they do You request, you have the right to submit again and in this case you will lose the previously obtained note.
It is understood that when talking about modules it refers to the global part and not to the internal subdivisions that these modules may have, because in them the criterion that the responsible professor thinks will be applied.
Not evaluable: Attendance at practical sessions (or field trips) is mandatory. The students will obtain the "Non-Appraising" qualification when the absence exceeds 20% of the programmed sessions. Therefore, in each module the student will be described as Non-Valuable, that without justified and documented cause does not attend this minimum of hours of programmed practical sessions and will be automatically fail with a note of 4,0 regardless of the global mark that they may obtain from the weighted calculation.
Bibliography
Animal and Plant Biology
LTABA, C. et al. 1991. Invertebrats no artròpodes. Història Natural dels Països Catalans. Vol. 8. Enciclopèdia Catalana. Barcelona.
ARMENGOL, J. et al. 1986. Artròpodes (I). Història Natural dels Països Catalans. Vol. 9. Enciclopèdia Catalana. Barcelona.
BARNES 2009. Zoologia de los Invertebrados. Ed. MacGraw-Hill. Interamericana. Setene edició.
BLAS, M.et al. 1987. Artròpodes (II). Història Natural dels Països Catalans. Vol. 10. Enciclopèdia Catalana. Barcelona.
BOLD, H.C. et al. 1989. Morfología de las plantas y los hongos. Omega. Barcelona.
BRUSCA R.C. i BRUSCA G.J. 2005. Invertebrados. Ed. MacGraw-Hill. Interamericana. Segunda edición.
FERRER, X et al. Ocells. Història Natural dels Països Catalans. Vol. 12. Enciclopèdia Catalana. Barcelona.
FOLCH, R. et al. 1984. Vegetació. Història Natural dels Països Catalans. Vol. 7. Enciclopèdia Catalana. Barcelona.
FONT i QUER, P. 1963. Diccionario de Botánica. Labor. Barcelona.
HICKMAN, C.P. et al. 2014. Integrated Principles of Zoology. Ed. Interamericana. Quinzena edició.
IZCO, J. et al. 2004. Botánica. McGraw-Hill-Interamericana. Madrid.
LLIMONA, X. (ed.) 1985. Plantes inferiors. Història Natural dels Països Catalans. Vol. 4. Enciclopèdia Catalana. Barcelona.
LLIMONA, X. (ed.) 1991. Fongs i líquens. Història Natural dels Països Catalans. Vol. 5. Enciclopèdia Catalana. Barcelona.
MASALLES, R.M. et al. (eds.) 1988. Plantes superiors. Història Natural dels Països Catalans. Vol. 6. Enciclopèdia Catalana. Barcelona.
MAUSETH, J. D. 1998. Botany. An Introduction to Plant Biology, 2/e. Multimedia enhanced edition. Jones & Bartlett Publ. Boston, Toronto, London, Singapur.
SOSTOA, A. et al. Peixos. Història Natural dels Països Catalans. Vol. 11. Enciclopèdia Catalana. Barcelona.
STRASBURGER, E. et al. 2004. Tratado de Botànica. Omega. Barcelona.
VIVES, J. et al. Amfibis, rèptils i mamífers. Història Natural dels Països Catalans. Vol. 13. Enciclopèdia Catalana. Barcelona.
Web sites
• Animal Diversity Web: http://animaldiversity.ummz.umich.edu/
• Adena/World Wildlife Found: http://www.wwf.es/
• Biodidac: http://biodidac.bio.uottawa.ca
• Tree of life web project: http://tolweb.org/tree/phylogeny.html
• Understanding evolution: http://evolution.berkeley.edu/evolibrary/article/evo_01
• Comissió Internacional de Nomenclatura Zoològica: http://www.iczn.org/
• Curs de Botànica de la Universitat d’Extremadura: http://www.unex.es/botanica/LHB
• Museu Nacional de Ciències Naturals de Madrid (CSIC): http://www.mncn.csic.es/
• Natural History Museum, Londres: http://www.nhm.ac.uk/
Plant Physiology
1) Fisiología Vegetal, J. Barceló et al., Ed. Piràmide, Madrid 2005 i següents
2) Plant Physiology, L. Taiz y E. Zeiger,Sinauer, Sunderland, MA (USA), 2006 i següents.
Web sites
Campus Virtual de l’Autònoma Interactiva: https://cv2008.uab.cat
Genetics
Benito, C. 1997. 360 problemas de Genética. Resueltos paso a paso. Editorial Síntesis, Madrid.
Griffiths, A.J.F., S.R. Wessler, R.C. Lewontin & S.B. Carroll. 2008. Genética. 9a. edició. McGraw Hill - Interamericana.
Jiménez Sánchez, A. 2008. Problemas de Genética para un curso general. Colección manuales UEX-52. Servicio de Publicaciones, Universidad de Extremadura.
Ménsua, J.L. 2003. Genética. Problemas y ejercicios resueltos. Pearson Prentice Hall.
Pierce. B.A. 2011. Fundamentos de Genética. Conceptos y relaciones. 1a. edició. Editorial Médica Panamericana.
Pierce. B.A. 2016. Genética. Un enfoque conceptual. 5a. edición. Editorial Médica Panamericana.
Organic Chemsitry
¨ G.H. Schmid. Química Biológica. Ed. Interamericana.1986.
¨ T. W. G. Solomons. Organic Chemistry (12a Ed.), John Wiley and Sons, New York, 2017 (o qualsevol edició anterior).
¨ K. P. C. Vollhardt, N. E. Schore. Organic Chemistry. Structure and Function (8a Ed.), W. H. Freeman and Co., New York, 2018 (o qualsevol edició anterior).
¨ Nomenclatura de Química Orgànica. Seccions A, B i C. Regles definitives, Institut d'Estudis Catalans, Barcelona, 1989.
¨ W. R. Peterson, Formulación y Nomenclatura en Química Orgánica, EUNIBAR, 1987.
Qualsevol llibre sobre Química Orgànica general serà útil per seguir aquesta matèria.
E-books
Software
- ChemSketch, ACDLabs. Free program for drawing structures. Link: https://www.acdlabs.com/resources/freeware/chemsketch/index.php
- Microsoft Excel
- Navegador web.
Language list
| Name | Group | Language | Semester | Turn |
|---|---|---|---|---|
| (PLAB) Practical laboratories | 411 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 412 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 413 | Catalan/Spanish | second semester | morning-mixed |
| (PLAB) Practical laboratories | 414 | Catalan/Spanish | second semester | morning-mixed |