Degree | Type | Year | Semester |
---|---|---|---|
2500252 Biochemistry | OB | 1 | 1 |
There are no prerequisites for this course. The student is advised to refresh the chemistry and biology knowledge acquired during the "batxillerat".
The general objective of this course is to familiarise the student with the basic techniques used in the biochemistry/molecular biology laboratory. This is a classroom-based course centred in theoretical aspects, which will be worked out in the lab in the Laboratori integrat I and II, scheduled later during the academic year.
Safety, good practices, laboratory notebook, use of materials and reagents, quantitative transfer of liquids, pipette use, statistical analysis, computer tools. Buffers, electrodes, biosensors. Sample preparation techniques.
Basic principles and instrumentation: Beer-Lambert law. UV, visible and fluorescence spectroscopy.
Applications, protein and nucleic acid quantification.
Basic principles.
Instrumentation. Types of centrifuges depending on their speed: low, high and ultracentrifugation. Rotor types.
Applications: fractional centrifugation for subcellular fractionation, gradient density centrifugation for the separation of blood coponents.
Analytical centrifugation.
Basic principles. Types and materials: Planaar and column chromatography. Gel filtration, ionic exchange, affinity. HPLC.
Basic types and applications.
Basic principles.
Electrophoretic methods and instrumentation. Supports: paper, agarose and polyacrylamide.
Applications: nucleic acid separation in agarose gels and polyacrilamide gel electrophoresis (PAGE) for protein separation. PAGE types.
Isolation and characterisation of DNA, restriction digestion of DNA. Nucleic acid amplification: the polymerase chain reaction (PCR). Basic principles of PCR and applications.
Antibody production, immunoelectrophoresis, Western blot, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, fluorescent activated cell sorting (FACS).
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Lectures | 25 | 1 | 3, 5, 4, 7, 6, 9, 8, 2, 10, 11 |
Type: Supervised | |||
Tutorial hours | 8 | 0.32 | 1, 3, 5, 4, 7, 6, 9, 8, 2, 10, 11 |
Type: Autonomous | |||
Autonomous learning: MOODLE activities | 20 | 0.8 | 1, 7, 6, 2, 10, 11 |
Self-study | 10 | 0.4 | 3, 5, 4, 6, 9, 8, 10, 11 |
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
MOODLE activities | 30% | 8 | 0.32 | 1, 7, 2, 10, 11 |
Problem solving test | 35% | 2 | 0.08 | 3, 5, 4, 6, 9, 8, 10, 11 |
Theoretical concepts test | 35% | 2 | 0.08 | 3, 5, 4, 6, 9, 8, 10, 11 |