Integrated Laboratory Class 2
Code: 100885 ECTS Credits: 3| Degree | Type | Year |
|---|---|---|
| Biochemistry | OB | 1 |
Contact
- Name:
- Maria Plana Coll
- Email:
- maria.plana@uab.cat
Teachers
- Andromeda Celeste Gomez Camacho
- Xavier Solans Monfort
- Ignasi Roig Navarro
Teaching groups languages
You can view this information at the end of this document.
Prerequisites
The student must attend simultaneously or have taken the theory subjects, which are taught during the same semester, corresponding to the contents of the practices of this subject,
In order to attend the laboratory classes it is necessary for the student to justify having passed the biosecurity and security tests that you will find in the Virtual Campus and be knowledgeable and accept the operating rules of the Bioscience Laboratories.
The test is answered in the corresponding space of the Virtual Campus and the information that must be consulted is in the communication space of the Degree in Biochemistry.
It is advisable for students to review the theoretical contents on which this subject is based
Objectives and Contextualisation
The subject of Integrated Laboratory 2 is part of a set of six subjects that are distributed throughout the first six semesters of the Degree in Biochemistry.
The educational objective of these subjects is the acquisition of practical skills of the student.
The contents are organized in a growing order of complexity, associated to the needs and to the acquisition of the theoretical contents.
During the Integrated Laboratory II the student acquires practical skills in the contents:
- Thermodynamics and Kinetics
- Physics
- Microbiology
- Organic Chemistry of Biochemical Processes
-- Basic instrumental techniques.
Laboratory internships focus on learning basic techniques specific to each field and on the characteristics of working in the laboratory.
Basic Instrumental Techniques Module
-Be able to choose and prepare the appropriate pH buffering system.
-Be able to make a colorimetric determination of the concentration of substances
-Be able to use chromatography in protein purification.
-Be able to perform electrophoresis on polyacrylamide gels as a common tool in the separation and identification of proteins.
Organic Chemistry Module of Biochemical Processes
Objectives:
Mastery of experimental techniques employed in organic chemistry laboratories, including reflux, liquid/liquid extraction, eight-duty filtration, purification by recrystallization, and oxidation/reduction (Redox) reactions.
Module Physics
Know how to apply basic techniques in the determination of physical parameters.
Module Microbiology
- Understand and know how to apply basic laboratory techniques to work experimentally with microorganisms.
- Know how to perform basic calculations to determine microbiological parameters.
- Evaluate the presence of microorganisms, their diversity and their ability to spread in all types of environments.
Learning Outcomes
- CM22 (Competence) Clearly and concisely describe experimental results in the field of biochemistry, considering options for improvement.
- CM23 (Competence) Work as a team when performing experiments and analysing their results.
- CM24 (Competence) Understand disposal methods for the different types of waste generated in a biochemistry or molecular biology laboratory.
- KM26 (Knowledge) Identify cell systems useful for experimentation in biochemistry and molecular biology.
- KM27 (Knowledge) Describe the theoretical foundations and instrumentation used in basic and advanced biochemistry.
- SM25 (Skill) Use digital resources to search for information, study biomolecules and calculate key parameters.
- SM26 (Skill) Interpret experimental results obtained using the main techniques of biochemistry.
- SM27 (Skill) Apply the techniques and methods for addressing cell culture techniques, classical genetics, immunological detection, recombinant DNA, separation, purification and analysis of biomolecules in the biochemical field.
Content
The subject is structured in:
Basic Instrumental Techniques
Contents
Practice 1 (2h).
Determination of glucose concentration by a colorimetric method.
Analysis of an absorption spectrum.
Practice 2 (2h).
Ice chromatography filtration: separation of hemoglobin from vitamin B12 and dextran blue.
Protein separation by electrophoresis in SDS. Preparation of the ice (which will be made to run the next day).
Practice 3 (2h).
Determination of the Mr of some proteins by SDS electrophoresis (Example: actin and myosin).
Physics
There will be 4 practices. Before carrying out the first practice, a brief introduction to the analysis of error in experimental measurements will be made.
Practice 1 (approximately 3 hours)
Determination of the viscosity coefficient of liquids using the Stokes method.
Practice 2 (approximately 3 hours)
Hooke's Law and Properties of Oscillatory Motion. Determination of the resonance frequency of an oscillatory system.
Practice 3 (approximately 3 hours)
Determination of the charge-to-mass ratio of electrons.
Practice 4 (approximately 3 hours)
Experimental consequences of the wave nature of light. Diffraction.
Thermodynamics and Kinetics
Contents
1. Use of the calorimeter to determine phase change and reaction enthalpies.
Determine the heat capacity of the calorimeter, using the mixtures method.
Measure the latent heat of melted ice and the reaction enthalpy of an acid-base neutralization.
2.- Kinetics of the reaction of methyl violet in basic medium.
To determine the pseudo velocity constant for the reaction of methyl violet in basic environment in excess of hydroxyl ions and at room temperature.
Determine the order of the reaction with respect to methyl hydroxide and violet and the velocity constant
Organic Chemistry Module of Biochemical Processes
Contents
PRACTICE 1.- SN1: SYNTHESIS OF 2-CHLORO-2-METHYLBUTANE FROM 2-METHYL-2-BUTANOL
Objectives: Mastery of the experimental techniques of crystallization, recrystallization, suction filtration, melting point determination and thin layer chromatography.
PRACTICE 2.- OXIDATION OF A METHYL GROUP TO CARBOXYL OBTAINING P-NITROBENZOIC ACID FROM p-NITROTOLELIENE
Microbiology module
Daily practical sessions of 3 hours each
Practice 1. Isolation, observation, characterization and identification of microorganisms
Practice 2. Methods of counting microorganisms
Practice 3. Ubiquity and microbial diversity
Practice 4. Kinetic growth of a microorganism
Activities and Methodology
| Title | Hours | ECTS | Learning Outcomes |
|---|---|---|---|
| Type: Directed | |||
| practice sessions in the laboratory | 56 | 2.24 | CM22, CM23, CM24, KM26, KM27, SM25, SM26, SM27, CM22 |
| Type: Supervised | |||
| Tutor sessions | 2 | 0.08 | KM27, SM25, SM26, SM27, KM27 |
| Type: Autonomous | |||
| Study | 7 | 0.28 | CM22, CM23, CM24, KM26, KM27, SM25, SM26, SM27, CM22 |
The practical sessions will be given in small groups of students (of about 20 per session) in the laboratory. They are designed to learn how to use the technical instrument and complement the theoretical training.
The attendance to the classes of this subject is obligatory since they imply an acquisition of competitions based on the practical work.
Before beginning a practical session the student must have read the protocol and know therefore the objectives of the practice, the foundations and the procedures that must be carried out. In the event that any calculation is required to do the practice in question, the student will have done them previously at the entrance to the laboratory.
If so, you must know the specific safety and waste treatment measures.
In the practical sessions you have to take:
- Protocol and, if applicable, the questionnaire.
- A notebook to collect the information of the experimental work.
- Laboratory baton.
- Safety glasses.
- Permanent marker
Microbiology module:
At the beginning of the subject the student will receive a Manual with the practical work that he will have to develop. This will be available on the Virtual Campus of the subject or where the teacher indicates.
These practices will be taught in three small groups of students, and they include 5 sessions of three hours each at the rate of one session per day during the whole week.
Attendance at the practical classes is mandatory in order to be able to acquire the competencies of the module. If a student, due to justified and unpredictable cause, has not been able to attend a practical session, he must speak with the responsible professor and present the corresponding supporting evidence as soon as possible. Health problems are deemed justified (the corresponding medical justification must be attached) or serious personal problems.
In order to be able to attend the practical classes of the laboratory,the student must pass the security test that will be found in the section Security in the Laboratories of the Virtual Campus of the faculty. In addition, you must comply with the regulations of work in a Microbiology laboratory that you will find indicated in the Manual itself. In each practical session, students must wear their own coat, protective goggles, lighter, permanent pen, calculator, a notebook to write down the observations made and the Practices Manual.
To carry out the practices the students will work in pairs and under the supervision of the teacher. At the beginning or during each daily session theteacher will make a brief theoretical explanation of the content of the practice and theexperiences to be carried out by the students, as well as the specific security measures and the treatment of the different chemical and biological waste generated. In order to achieve good performance and acquire the competencies corresponding to this activity, it is essential that the student read a comprehensive reading of the Manual protocols before their completion.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Assessment
Continous Assessment Activities
| Title | Weighting | Hours | ECTS | Learning Outcomes |
|---|---|---|---|---|
| Basic instrumental technics. Question answers | 13 | 1 | 0.04 | CM22, CM23, CM24, KM26, KM27, SM25, SM26, SM27 |
| Microbiology | 25 | 2 | 0.08 | CM22, CM23, CM24, KM26, KM27, SM25, SM26, SM27 |
| Organic Chemistry of Biochemical Processes. Test | 22 | 3 | 0.12 | CM22, CM23, CM24, KM27, SM25, SM26, SM27 |
| Physics | 25 | 2 | 0.08 | CM23, CM24, KM26, KM27, SM25, SM26, SM27 |
| Thermodinamics and Kinetics | 15 | 2 | 0.08 | CM23, CM24, KM26, KM27, SM25, SM26, SM27 |
Basic Instrumental Techniques
The practices will be evaluated taking into account:The resolution of questionnaires with a weight of 70% of the grade of the module in which it will be evaluated:• The understanding of the fundamentals of experimental methods.• The ability to process and analyze experimental data.• The ability to interpret experimental results. The follow-up of the experimental work in the laboratory in which it will be evaluated with a weight of 30% of the grade of the module:• The previous preparation work, especially in those practices that require prior calculations.• The application of the general rules of safety and operation of a laboratory.• The application of waste disposal processes.• The ability to work in a team.
Physics
The evaluation will be carried out through the preparation of practice reports of practices 1 and 2.
The final evaluation of this part will be obtained from the weighted average of the evaluation of the two internships.
Thermodynamics and Kinetics
The evaluation will be carried out through i) evaluation of the preparation of the practices through a test where it is tried to verify that the students have adequately prepared the practices that they must carry out (25%); behavior and attitude. (10%); rating of reports (65%).
Organic Chemistry of Biochemical Processes
The final grade of the subject will be the result of 60% of the mark of the 40% exam of the continuous assessment of the practice professors.
The minimum mark of the exam to be able to pass the subject will be 3,5 out of 10.
The theoretical exam will be held on the last day of internships.
In order to pass the course, it is not possible to miss more than one day in the laboratory, as long as a receipt is received.
Microbiology module:
In this module there will be twotypes of evaluation activities:
1- Continuous assessment of work as a couple.
The students must submit a report of the results obtained that will consist of filling out a dossier that the professor will have distributed previously.
This report will be included in the last practice session.
2- Individual evaluation of the contents.
A questionnaire will be conducted on the last day of practice, which will consist of answering 15 questions test and solving a practical exercise.
These assessment activities will have a weight of 3 and 7 points, out of 10, respectively.
In addition, the attitude and work of the student in the laboratory will be taken into account (punctuality, correct use of the laboratory equipment (mainly the robe), compliance with the safety regulations and understanding and follow-up of the Manual of the subject). This assessment does not entail an increase in the note, but it can mean the reduction of up to 20% of the final grade obtained in this module.
To pass the Microbiology Module, a minimum score of 3.5 must be obtained. Otherwise, the final maximum grade of the subject will be 3.5.
Since attendance at the practical sessions is mandatory, the absence of any of the sessions must be justified and may not exceed 20%. In case this value is exceeded, the module will be qualified with a Non-Valuable.
Final grade
The final qualification of the subject will be obtained from the weighted average of the qualification of the different ones
Contents: 16% Animal Histology, 21.5% Biochemistry, 14% Thermodynamic and Kinetic, 21.5% Organic Chemistry of Biochemical Processes and 27% Microbiology.
To pass the subject, at least 80% of the programmed sessions must be attended, obtain a final grade of 5 or more and obtain a minimum qualification of 3.5 in each group of contents. Students who do not achieve the minimum qualification of 3.5 in one or more of the groups of contents will receive a maximum final grade of the subject of 3.5 points.
The student will obtain the non-valuable qualification when he has attended less than 20% of the programmed sessions.
Single assessment
For those students who take the single assessment is compulsory to do the laboratory practices (PLAB) in the scheduled sessions with the rest of the group.
The single assessment consists of a single synthesis test with questions from all the integrated laboratory modules on the day scheduled in the academic calendar. The grade obtained in the synthesis test is 75% of the final grade of the subject. The attitude during the practices and attendance will be the remaining 25%.
The same retake system will be applied as for the continuous assessment.
Bibliography
Organic Chemistry Module of Biochemical Processes
► D. L. Pavia, G. M. Lampman I G. S. Kriz Jr. Introduction to Organic Laboratory Techniques (3ª Ed.), Saunders, Philadelphia, 1988. ►
M. P. Cava, M. J. Mitchell. Selected Experiments in Organic Chemistry, Benjamin, New York, 1966.
►J. W. McFarland. Organic Laboratory Chemistry, Mosby, St. Louis, 1969.
► L. M. Harwood, C. J. Moody. Experimental Organic Chemistry: Principles and Practice, Blackwell Scientific Publ., Oxford, 1989.
► Vogel Text Book of Practical Organic Chemistry, Vogel’s (5ª Ed.) revisada per B. S. Furniss, A. J. Hannaford, P. W. G. Smith, A. R. Tatchell, Lognman, Essex, 1989.
Basic Instrumental Techniques
GelAnalyzer 19.1 (www.gelanalyzer.com) by Istvan Lazar Jr., PhD and Istvan Lazar Sr., PhD, CSc
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH Image to ImageJ: 25 Years of Image Analysis. Methods of Nature, 9(7), 671–675. doi:10.1038/nmeth.2089
Excel: microsoft.com
For other modules specific bibliography is includes in the teaching guide
Software
No specific software is required
Groups and Languages
Please note that this information is provisional until 30 November 2025. You can check it through this link. To consult the language you will need to enter the CODE of the subject.
| Name | Group | Language | Semester | Turn |
|---|---|---|---|---|
| (PLAB) Practical laboratories | 311 | Catalan | second semester | morning-mixed |
| (PLAB) Practical laboratories | 312 | Catalan | second semester | morning-mixed |
| (PLAB) Practical laboratories | 313 | Catalan | second semester | morning-mixed |