Research Methods in Pre-clinical Pharmacology
Code: 42361 ECTS Credits: 9| Degree | Type | Year |
|---|---|---|
| 4311309 Pharmacology | OT | 0 |
Contact
- Name:
- Alheli Rodriguez Cortes
- Email:
- alheli.rodriguez@uab.cat
Teachers
- Carles Cristòfol Adell
- Àgueda Flores Flores
- Gianluigi Caltabiano
- Miguel Angel Vicente Martínez
- Alheli Rodriguez Cortes
- (External) A. Guzmán
- (External) A. Norniella
- (External) A. Pujol
- (External) D. López
- (External) G. Encina
- (External) J. Alfons
- (External) J. De la Puente
- (External) J. Hernández
- (External) J. L. Montero
- (External) J.M. Cendros
- (External) JL Ruiz
- (External) L. Canut
- (External) L. Gimenez
- (External) M. Aulí
- (External) M. Aulí
- (External) N. Prats
- (External) R. Mollà
- (External) R. Roca
Teaching groups languages
You can view this information at the end of this document.
Prerequisites
There are no special prerequisites.
Objectives and Contextualisation
To apply basic pharmacological and methodological knowledge to develop preclinical drug research and interpret the results.
Competences
- Design and conduct research on drugs.
- Generate innovative ideas.
- Interpret one's own and other results of research on drugs.
Learning Outcomes
- Apply models to describe and predict the time course of drug concentrations and their pharmacological response.
- Generate innovative ideas.
- Interpret models to describe and predict the time course of drug concentrations and their pharmacological response
- Plan, design, use and interpret studies (not on humans) with the aim of evaluating the effects of drugs and their mechanism of action.
- Plan, design, use and interpret studies (not on humans) with the aim of evaluating the time course of drug concentration in biological tissues and its relation to the effects observed.
- Plan, design, use and interpret studies (not on humans) with the aim of evaluating toxicity and possible adverse reactions.
- Recognise and apply the ethical and quality requirements for conducting non-clinical research with drugs.
Content
a) Evaluation and validation of a pharmacological target. Molecular screening: automation and high performance. Levels of pharmacological research: computer (in silica), cells, organs, animals, humans. In vitro studies: molecular for the evaluation of targets, cell cultures (biochemical and molecular), isolated organs (physiological, biochemical and molecular). Ex vivo studies. Methodologies used in in vivo studies: conscious animal and anesthetized animal (measurement of blood pressure). Animal models of diseases induced by administration of compounds, by surgical intervention or by genetic manipulation. Methodologies for studies of psychotropic drugs.
b) In silico studies. In vitro studies: physical-chemical characterization (release, dissolution). In vitro studies: metabolism. In vivo studies: handling of animals, choice of species. In vivo studies: design. Microdialysis techniques. Plasma protein binding. Animal / human extrapolation (allometry): data analysis. Toxicokinetics and special pharmacokinetics.
c) Mechanisms of toxicity. In vitro toxicity studies. In vivo toxicity studies: single administration. In vivo toxicity studies: repeated administration. Toxic-anatomopathology. Reproductive toxicology: infertility, teratogenicity (embryotoxicity). Genotoxicity Carcinogenesis Phototoxicity Immunotoxicity Local tolerability Behavior tests
d) Ethics of animal research. Ethical committees BPLs: Good Laboratory Practices. Guidelines for pre-clinical research of Regulatory Agencies. Legislation. Preclinical information for registration. Study of patent situation.
e) Models, development strategies / techniques: empirical, mechanistic. Theory of statistical moments. Convolution / deconvolution: theory and applications. Non-linear adjustment. Population approximation. Sensitivity analysis and biases: strategies and applications.
Activities and Methodology
| Title | Hours | ECTS | Learning Outcomes |
|---|---|---|---|
| Type: Directed | |||
| Classroom practices (PAUL) | 11 | 0.44 | 1, 2, 3, 5, 4, 6, 7 |
| Clinical case seminars (SCC) | 4 | 0.16 | 1, 2, 3, 5, 4, 6, 7 |
| Master Class (TE) | 50 | 2 | 1, 2, 3, 5, 4, 6, 7 |
| Practical Experimental Laboratory (PLAB) | 3 | 0.12 | 1, 2, 3, 5, 4, 6, 7 |
| Type: Supervised | |||
| Review of papers | 24 | 0.96 | 1, 2, 3, 5, 4, 6, 7 |
| Unscheduled tutorials | 3 | 0.12 | 1, 2, 3, 5, 4, 6, 7 |
| Type: Autonomous | |||
| Study, written works ... | 127 | 5.08 | 1, 2, 3, 5, 4, 6, 7 |
The final grade is the arithmetic mean of the grades obtained in the subjects that compose it.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Assessment
Continous Assessment Activities
| Title | Weighting | Hours | ECTS | Learning Outcomes |
|---|---|---|---|---|
| Attendance and participation | 10 % | 0 | 0 | 1, 2, 3, 5, 4, 6, 7 |
| Oral presentations | 42,5 % | 2 | 0.08 | 1, 2, 3, 5, 4, 6, 7 |
| Short questions exam | 47,5 % | 1 | 0.04 | 1, 2, 3, 5, 4, 6, 7 |
The final grade is the arithmetic mean of the grades obtained in the subjects that compose it.
Bibliography
Updated bibliography will be provided for ech subject.
General bibliography
- Burton LL, Lazo JS, Parker KL. Goodman & Gilman. Las Bases Farmacológicas de la Terapéutica. 13a Edición. Madrid: McGraw-Hill Interamericana de España S.L., 2018.
- Doménech J, Martínez J, Plá JM. Biofarmacia y Farmacocinética Volumen I: Farmacocinética. Madrid: Editorial Síntesis, 2000.
- Fundación Genoma España. Guia de desarrollos preclínicos. Genoma España. 10 aniversario (2002-2012). https://www.agenciasinc.es/Noticias/Genoma-Espana-publica-una-guia-de-desarrollos-preclinicos
- Kenakin TP. Pharmacology in Drug Discovery and Development. 2nd Edition. London: Academic Press, 2016.
- Lorenzo P, Moreno A, Leza JC, Lizasoain I, Moro MA, Portolés A. Velazquez. Manual de farmacologia basica y clinica. Madrid: Editorial Médica Panamericana, 2021.
- Rosenfeld GC, Loose DS. Pharmacology. 4th Ed. Baltimore: Lippincott Williams & Wilkins, 2006.
- Weisman RS, Smith C, Goldfrank LR. Toxicokinetics. Applying pharmacokinetic principles to the poisoned patient. In: Hoffman RS, Goldfrank LR eds. Critical Care Toxicology (Contemporary management in critical care). New York: Churchill Livingstone, 1991 (vol 1; no 3): 21-42.
- Young-Jin S, Shannon M. Pharmacokinetics of Drugs in Overdose. Clinical Pharmacokinetics 1992; 23 (2): 93-105.
Software
No special software is required.
Language list
| Name | Group | Language | Semester | Turn |
|---|---|---|---|---|
| (PAULm) Classroom practices (master) | 101 | Catalan/Spanish | first semester | afternoon |
| (PLABm) Practical laboratories (master) | 101 | Catalan/Spanish | first semester | morning-mixed |
| (SCCm) Clinical case seminars (master) | 101 | Catalan/Spanish | first semester | morning-mixed |
| (TEm) Theory (master) | 101 | Catalan/Spanish | first semester | afternoon |