Degree | Type | Year |
---|---|---|
2500890 Genetics | OB | 3 |
You can view this information at the end of this document.
The Integrated Laboratory VI is the sixth course in a set of 6 which are distributed along 6 semesters of the first three courses of the degree of genetics. These subjects aim to give a solid foundation of experimental procedures, techniques and skills of genetics and related sciences. The practical work help to reinforce the theoretical concepts acquired in the theory, and allow us to understand the essential dialogue between theory and experimentation that have given rise to the body of knowledge that constitutes the science of genetics.
The Integrated Laboratory VI has as objectives the acquisition of experimental skills in 3 specific modules of content:
Module of Molecular genetic diagnosis
The practices are structured in 4 sessions of 4 hours each during which different molecular genetics techniques will be performed in order to reach the diagnosis of a genetic disease (we will take as an example the study of Spinal Muscular Atrophy).
The objectives of the practices are:
The global analysis of the different results will allow us to reach the molecular diagnosis of a family with Spinal Muscular Atrophy.
The four practical sessions are structured as follows:
4. Resolution of the case
4.1. Interpretation of microsatellite markers
4.2. Resolution of the case
4.3. Writing final report
Module of Evolution
The main objective of the Evolution module is to help the student to learn to apply the comparative method in evolutionary inference by using molecular sequences. The module includes the processes of hypothesis formulation and testing, obtention of sequence data for their alignment, modeling of the process of evolutionary change of the sequences, and application of methods of i) phylogenetic reconstruction, ii) characterization of the evolution of gene families, and iii) Identification of footprints of molecular adaptation. In addition, a perspective on the levels and structure of genetic variation in Homo sapiens will be provided by comparison with what is observed in other closely related living and extinct species. Through these practices the student will acquire skills in the application of analytical techniques to biological and molecular data.
Module of Systems biology
The main objective of the module is to learn how to use the right software in systems biology and better understand the behavior of biological systems. To achieve this the student will conduct simulation exercises with computer to see how the behaviorof a biological system cannot be predicted from the behavior of itsisolated components.
Module of Molecular genetic diagnostic
The four practical sessions are structured as follows:
4. Resolution of the case
4.1. Interpretation of microsatellite markers
4.2. Resolution of the case
4.3. Writing final report
Module of Evolution
a) Alignment: obtention, reliability and interpretation.
b) Modeling of molecular evolution: problem of multiple substitutions and selection of optimal models of sequence evolution.
c) Molecular phylogenetic trees: obtention, reliability and interpretation.
d) Distribution of character states. Evolution of gene families.
e) Molecular dating. Human genetic diversity
Module of systems biology
The module includes practical exercises corresponding to the theory of the subject ' systems biology' and will consist in the simulation of systems for example corresponding to genetic, metabolic networks, signal transmission or of more complex systems, developed by the student with the recommended software or downloaded from databases of models according to the case.
In general the practices include exercises for the use of software and databases specific to each of the topics. These exercises will allow.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Evolution Module | 15 | 0.6 | 7, 9 |
Module Molecular Systems Biology | 15 | 0.6 | 2, 4, 9 |
Module Molecular genetic diagnosis | 16 | 0.64 | 1, 3, 4, 5, 6, 7, 8, 10, 11, 14 |
Type: Supervised | |||
Individual tutorials | 1 | 0.04 | |
Type: Autonomous | |||
Lab booknote | 3 | 0.12 | |
Study | 23 | 0.92 |
The subject is taught in small groups of students (maximum 20 per session) in the laboratory or computer rooms. Students have a manual or practice guide for each Module. It is necessary to read the corresponding part of each session carefully before starting the practice to obtain the maximum advantage. Students will have to attend the assigned group of internships obligatorily. Only occasional changes will be accepted as long as they are balanced (a student from a group for a student from another group). If a student has not been able to perform a practice session with his group, he can retrieve it by attending another group, as long as the group in question has free spaces.
Molecular genetic diagnosis
The students will have a detailed guideine of the practices with the protocols and the detailed information to be able to carry out them efficiently with the support of the teacher. The guideline will be available on the Virtual Campus of the subject. The students will not only work their data, but will analyze and interpret the set of results obtained.
Evolution
The Practicum will take place in computer rooms using several applications. Data obtained from scientific publications will be used. Different concepts and hypotheses about the evolutionary process will be illustrated through presentation of real problems. A guide to the Practicum will be available on the Virtual Campus of the subject.
Systems biology
Systems biology practices will be carried out in the computerrooms on days and hours defined in the teaching calendar.
For each practice, the student will find the practical guidelines on the virtual campus of the subject 'Systems Biology'.
The student will carry out the practice following the practice guideline and save the files generated in the folder of his personal record provided by the UAB.
When finishing the practice, the student will deliver, through the virtual campus, the files of the exercises as described in each practice.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Evolution Module | 33% | 0.7 | 0.03 | 4, 9, 12 |
Module Molecular biology of systems. Continuous evaluation of the results worked | 33% | 0.65 | 0.03 | 2, 4, 9 |
Module Molecular genetic diagnosis. Continuous evaluation of the results worked | 33% | 0.65 | 0.03 | 1, 3, 4, 5, 6, 7, 8, 10, 11, 13, 14 |
Attendance to practical sessions is mandatory. Students missing more than 20% of programmed sessions will be graded as "No Avaluable".
Attendance to the practices is mandatory and therefore an absence without justification may entail the non-evaluation of one or more modules. Missing a session implies a reduction of the grade equal to% of this session in the whole module. Thus, in a module of 4 sessions, missing a day involved a reduction of 25% of the note in this module. Those students who can not attend the session of their group for just cause are exempt from this penalty. Health problems are deemed justified (the corresponding medical certificate must be carried out by the practice coordinator) or serious personal problems. In this case the practice will be recovered whenever possible.
Notwithstanding other disciplinary measures deemed appropriate, and in accordance with the current academic regulations, the irregularities committed by the student who can lead to a variation in the qualification of an act of self- evaluation. Therefore, copying or letting copy a practice or any other evaluation activity will imply suspending it with a zero, and if it is necessary to pass it to pass it, the entire subject will be suspended. Qualified assessment activities will not be recovered in this way and by this procedure, and therefore the subject will be suspended directly without opportunity to recover it in the same academic year.
Module Molecular genetic diagnosis
This module will be evaluated by means of a final exam about the experimental procedure of the practices, that will take place the last day of the practices and will count 40% of the note. The other 60% of the mark will be evaluated by delivering a practical script. In the event that the student suspends the examination, the exam may be recovered, but the maximum grade that can be obtained in the recovery will be 5. The possibility of notifying notes is contemplated. The delivery note can not be recovered in any case.
Evolution Module
The module will be evaluated with a practical test of the contents included in the module. The final qualification of the module will take into account the attitude and work of the student in the classroom.
Systems Biology
Grading of the Systems Biology module will be calculated as follows:
First part of the grade (40%) will be obtained from practical sessions 1 to 4. It will be obtained from the responses to the proposed questionnaire as well as from the proposed exercises as described in each guide for the practical work.
The second part of the grade (60%) will be obtained from the 5th practical class (last one) from the delivered simulator files and the answers provided to the proposed questions, delivered in the summary document for the 5th practical class.
In case the weighted average obtained using the above procedure would not be equal or higher to 5, the student can take a remedial exam.
The final grade is the average of the notes of each module.
The scripts of the different practices will contain the specific bibliography of each one of them.
- COPASI (http://copasi.org/)
Name | Group | Language | Semester | Turn |
---|---|---|---|---|
(PLAB) Practical laboratories | 631 | Catalan/Spanish | second semester | morning-mixed |
(PLAB) Practical laboratories | 632 | Catalan/Spanish | second semester | morning-mixed |
(PLAB) Practical laboratories | 633 | Catalan/Spanish | second semester | morning-mixed |