Bioreactors
Code: 101022 ECTS Credits: 3| Degree | Type | Year |
|---|---|---|
| 2500502 Microbiology | OB | 3 |
Contact
- Name:
- Javier Garcia Ortega
- Email:
- xavier.garcia@uab.cat
Teaching groups languages
You can view this information at the end of this document.
Prerequisites
No specific requirements are defined for this course
Objectives and Contextualisation
- Knowledge acquisition about relevant aspects related to bioindustrial processes, such as mass balances, bioreactor design and its proper utilization, depending on its specific application.
- Learning about the main bioreactor types, their basic features and their main applications, for both enzymatic and microorganism based processes.
- To study the required elements to design and operate a bioreactor, such as conventional kinetic equations and deign equations, the interaction between the kinetic law and the operation mode, the mixing and aeration hardware, as well as the instrumentation and basic control elements. To analyze the ideal rector types, and from those to determine the requirements for the utilization of real reactors.
Learning Outcomes
- CM15 (Competence) Evaluate the role of microorganisms in processes of economic interest as producers of key compounds in the development of our societies and in the improvement of the quality of life.
- CM16 (Competence) Propose microbial processes to assess the environmental impact of human activity, as indicators of ecosystem alteration, as well as to recover contaminated environments.
- KM23 (Knowledge) Identify the operations and production processes involving microorganisms or their components.
- SM22 (Skill) Manage specific bibliography and internet tools to develop an academic work within the field of environmental or industrial microbiology both in English and in one's own language or others.
- SM23 (Skill) Select the appropriate methodologies to characterise populations and communities of microorganisms from environmental and industrial samples and their abiotic environment.
- SM24 (Skill) Analyse industrial operations and processes involving microorganisms or their components in order to contribute to their improvement and guarantee their success.
Content
UNIT 1.- BIOCHEMICAL ENGINEERING AND BIOTECHNOLOGY.
- The bioreactors in the biotechnological processes.
- Biotechnology, basic definitions
- Productive process and position of bioreactors
- Enzymatic and microbial kinetic models
- Definitions
- Microbial growth stoichiometry
- Celular growth, substrate consumption and product obtention.
- Microbial growth kinetic models
- Effects of the physico-chemical parameters on the enzymatic activities and on the microbial growth
- Mass and energy balances
- Mass and energy conservation principle.
- Mas balances in bioreactors.
UNIT 2.- IDEAL BIOREACTORS
- Basic bioreactor design
- Classifying bioreactors
- Ideal bioreactors: continuous and batch operation.
- Fed-batch operation. Systems with recirculation. A series of reactors.
UNIT 3.- REAL BIOREACTORS
- Typical configurations and bioreactor elements.
- Real bioreators: examples
- Non ideal flow
- Advanced Bioreactors
UNIT 4.- OPERATION, INSTRUMENTATION AND CONTROL OF BIOREACTORS.
- Aeration
- Oxygen transfer.
- Aeration and its eficiency.
- Determination of the kLa coefficient.
- Mixing
- Fermentation browth reology
- Shear stress effects.
- Mixers.
- Mixing and aeration.
- Bioreactor scale-up
- Control and instrumentation:
- Definitions
- Needs and incentives
- Elements of a control system
- Implementation of a control system: pH control, temperature control and dissolved oxygen
Activities and Methodology
| Title | Hours | ECTS | Learning Outcomes |
|---|---|---|---|
| Type: Directed | |||
| Lectures | 20 | 0.8 | CM15, CM16, KM23, SM22, SM24 |
| Workshops | 3 | 0.12 | CM15, CM16, KM23, SM22, SM24 |
| Type: Supervised | |||
| Team working | 10 | 0.4 | CM15, CM16, KM23, SM22, SM24 |
| Type: Autonomous | |||
| Study | 38 | 1.52 | CM15, CM16, KM23, SM22, SM24 |
LECTURES (20h): lectures will introduce the basic concepts of the course contents. If possible interactive audiovisual material to assist concept understanding.
WORKSHOPS (3h): workshops have as aim to strengthen the theoretical concepts with representative practical cases. Realistic use of bioreactors for production process with be targeted. Discussion and group working will be favored.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Assessment
Continous Assessment Activities
| Title | Weighting | Hours | ECTS | Learning Outcomes |
|---|---|---|---|---|
| Evaluation 1 (units 1 and 2) | 45% | 2 | 0.08 | CM15, CM16, KM23, SM24 |
| Evaluation 2 (units 3 and 4) | 45% | 2 | 0.08 | CM15, CM16, KM23, SM24 |
| Resoluction of an applied case | 10% | 0 | 0 | CM15, CM16, KM23, SM22, SM23, SM24 |
- GRADES :
Through(i) two individual written tests (defined as Tests 1 and 2 in the calendar) combining development questions with application of acquired concepts to practical cases; (ii) the resoluction of an applied case). For the students either who have not passed the course or did not attend the day of the tests, there will be a last written test (indicated as Recuperation in the calendar) combining development questions with application of acquired concepts to practical cases. Additionally, students who have passed the course willing to improve their grades can also use the retake process. They will do the whole test and the grade will be obtained with the recuperation test (retake process).
To be eligible for the retake process, the student should have been previously evaluated in a set of activities equaling at least two thirds of the final score of the course or module. Thus, the student will be graded as "Not Evaluable" if the weighting of all conducted evaluation activities is less than 67% of the final score.
The course does not include the possibility of single evaluation process.
Additional aspects:
- To pass the course it is required a mark of at least 5/10, either through the average of the evaluaction activities or from the recuperation test. To allow average of the evaluations activities (with the ponderation indicated in the table)
- Only failed individual tests require to go through the retake process (hence, individual tests reduce contents of the course).
- A student cannot be evaluated (i.e. marked as "No avaluable") when the set of evaluating activities done was lower than two thirds of the total mark of the course.
Bibliography
Doran, P.M. Principios de Ingeniería de los Bioprocesos. Acribia. (1998) [https://www-sciencedirect-com.are.uab.cat/science/book/9780122208515]
Bailey, J.E., Ollis, D.F. Biochemical Engineering Fundamentals. McGraw Hill. (1986)
Blanch, H.W., Clark, D.S. Biochemical Engineering. Marcel Dekker. (1997)
Gòdia, F., López, J. Ingeniería Bioquímica. Síntesis. Madrid. (1998)
Kosaric, N., Pieper, H.J., Senn, T., Vardar-Sukan, F., “The Biotechnology of Ethanol”, Wiley (2001)
Levenspiel, O. “Ingeniería de las reacciones químicas”, Wiley (2004)
Ollero de Castro, P.; Fernández Camacho, E. "Control e instrumentación de procesos químicos". Editorial Síntesis. (1997)
Vogel, H.C., Todaro, C.L. “Fermentation And Biochemical Engineering Handbook”, Noyes Publications (1997)
Software
During lectures as well as for the proposed tasks the use of EXCEL is widespread.
The optional assignment proposed in the course uses (free) specific software AQUASIM, whose use will be introduced during the course and provided for its use.
Language list
| Name | Group | Language | Semester | Turn |
|---|---|---|---|---|
| (PAUL) Classroom practices | 731 | Catalan | first semester | morning-mixed |
| (TE) Theory | 73 | Catalan | first semester | morning-mixed |