Degree | Type | Year |
---|---|---|
2500252 Biochemistry | OB | 3 |
You can view this information at the end of this document.
There are no official prerequisites. However, it is assumed that the student has acquired the basic knowledge of Molecular Biology explained in previous subjects of the degree of Biochemistry.
Recombinant DNA technology includes diferents methodologies developed from 1970-1980. These methodologies are now a basic tool in many biochemistry laboratories and have allowed in recent years a very important advance in the knowledge of the structure and function of biomolecules. In this subject the fundamentals of this technology will be presented. The general objective of the subject is to provide the knowledge that allows the student working with these methodologies during his professional future.
Specific objectives of the course:
Unit 1. Introduction od recombinant DNA technology. Basic Tools of Recombinant DNA: restriction enzymes, polymerases, exonuclases, ligases, reverse transcriptase. cDNA synthesis, CRISPR system.
Unit 2. Hybridization techniques. Tm concept. DNA and RNA Labelling. Southern, Northern blot and their applications. Dot-Blot. in situ hybridization. Fish. Microarrays.
Unit 3. Polymer chain reaction (PCR) Introduction. Design and optimization of the reaction. RT-PCR. Quantitative PCR (real-time PCR). Applications.
Unit 4. Molecular Cloning. Blunt and cohesive extreme. Adaptors and Linkers. DNA Ligation. Bacterial transformation. Detection of recombinant clones. Characteristics of the cloning vectors: plasmids and bacteriophages. Some examples of plasmid. Specific vectors by alternative cloning systems: recombination integration systems, topoisomerase-based cloning systems.
Unit 5. Libraries of cDNA versus RT-PCR/RNA-seq. Strategies for the construction of libraries, concept of abundance and complexity of mRNA. Synthesis of cDNA. Main vectors used in the construction of cDNA libraries. Screening of cDNA libraries. RT-PCR / RNA-seq as an alternative to cDNA libraries.
Unit 6. Libraries for genomic sequencing. Construction and screening of genomic llibraries versus high throughput genomic sequence. Concept of Representativeness. Strategies for obtaining libraries for genomic sequencimg. Vectors used in genomic llibraries: Lambda, Cosmids, BACS. Screening of genomic DNA libraries. " Genomic Walking" and/or obtention of probe (reverse PCR). High throughput sequencing technologies.
Unit 7. Expression of recombinant proteins in E. coli. Kind of vectors used. Optimization of recombinant protein expression. Fusion proteins. In vitro translation systems. Site-directed mutagenesis vs Molecular evolution (Phage display).
Unit 8. Cloning in Yeast (S. cerevisae). Transformation. Typology of vectors. Expressions of recombinant proteinsin yeast. "Two-hybrid" method for detecting protein-protein interactions.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Magisterial class | 16 | 0.64 | 2, 3, 4, 5, 7 |
Problem class | 8 | 0.32 | 2, 10 |
Type: Supervised | |||
Tutorials | 5 | 0.2 | 2, 3, 4, 5, 7, 10 |
Type: Autonomous | |||
Autonomous resolution of problems | 15 | 0.6 | 2, 3, 4, 5, 10 |
Autonomous study (teoric class) | 27 | 1.08 | 2, 3, 4, 7 |
The activities consist of theory and problem sessions.
Theory sessions
The teacher will explain the content of the syllabus with the support of audiovisual material that will be available to students in the Virtual Campus of the subject
Problem sessions
There will be 8 problem sessions per group. For these sessions, the theory group will be divided into two subgroups (A and B), the lists of which will be made public at the beginning of the course. Students will attend scheduled sessions for their group. At the beginning of the semester, a dossier will be delivered through the Virtual Campus with the problem statements, which will be solved by the teacher in a reasoned way and, if necessary, complementing part of the subjects explained in the theory classes.
Two of the problem sessions will be held in computer rooms in the afternoon. Each subgroup A and B will be divided into two (A1, A2, B1 and B2) and the sessions will take 2 hours.
Tutorials
Individual or small group tutorials will be held at the student's request. The objective of these tutorials will be to solve doubts, to orientate on the sources of information consulted and the preparation of the seminars. In case the number of requests is extremely high, especially before exams, a classroom tutorial could be held before exams, to solve doubts or to review basic concepts, which will be announced in due time through the Virtual Campus. These sessions will not be expository nor will the official syllabus be advanced, but will be sessions of debate and discussion.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Individual written test of problems | 25 % | 1.5 | 0.06 | 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 |
Theory module evaluation: multiple choice questions | 40% | 1 | 0.04 | 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 |
Written theory test of short questions | 35% | 1.5 | 0.06 | 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 |
In order to evaluate the level of assimilation of knowledge, as well as the capacity to relate concepts, critical reasoning and other transversal competences, a series of evaluative activities of different typologies will be carried out.
These evaluative activities will be identical for both the continuous evaluation system and the single evaluation system.
Continuous evaluation
Evaluation of the theory module (75%):
It will consist of two evaluative activities of different typologies.
1) Individual evaluation by means of pot-type questions (multiple-choice).
2) Individual evaluation by means of short open-ended questions relating several sections of the program, on the use of knowledge to interpret experimental results or to propose the most appropriate techniques to reach the objectives set out in the question.
There will be no minimum grade to make the average between the two types of evaluative activities and to obtain the final grade of the theory module.
The overall weight of the theory module will be 75% of the final grade of the course, and the test will be given on the date scheduled in the calendar.
This test may be recovered on the day set for the recovery of the subject
Evaluation of problems module (25%).
An individual evaluation will be carried out by means of a written test. It will consist of the resolution of 2 problems posed by the teacher of the same type as those worked during the formative activity of problems.
The weight of this test will be 25% of the final grade of the course and will be done on the date scheduled in the calendar.
This test may be recovered on the day set for the recovery ofthe subject.
Single Evaluation
Theory module evaluation (75%):
The single evaluation test of this module will be the same as in the continuous evaluation and will be done coinciding with the same date set in calendar as in the continuous evaluation.
The same recovery system will be applied as for the continuous evaluation.
Problem module evaluation (25%).
The written test of single evaluation of this module will be the same and will have the same weight as in the continuous evaluation (25%).
It will coincide with the same date fixed in the calendar as in the continuous evaluation, and the same recovery system will be applied as in the continuous evaluation.
General considerations for the two continuous and single evaluation systems
1) Gene Cloning and DNA Analysis : An Introduction T. A. Brown;T. A. Brown eBook | 2016 https://bibcercador.uab.cat/permalink/34CSUC_UAB/15r2rl8/cdi_askewsholts_vlebooks_9781119072553
2) MOLECULAR BIOTECHNOLOGY, PRINCIPLES AND APPLICATIONS OF RECOMBINANT DNA Harris, Bernadette;Harris, BernadetteeBook eBook | 2018. https://bibcercador.uab.cat/permalink/34CSUC_UAB/15r2rl8/cdi_askewsholts_vlebooks_9781683673101
3) Nicholl, Desmond S. T. An Introduction to genetic engineering 2008. eBook 2n edition (2002). https://bibcercador.uab.cat/permalink/34CSUC_UAB/15r2rl8/cdi_proquest_miscellaneous_18821168
4) S. B. Primrose and R. M. Twyman Principles of gene manipulation and genomics /, SEVENTH EDITION, eBook | 2006. https://bibcercador.uab.cat/permalink/34CSUC_UAB/15r2rl8/cdi_askewsholts_vlebooks_9781444309096
5) H. Freeman. Recombinant DNA : genes and genomes - a short course, 2007
6) J. Perera, Julián. Ingeniería genética 2002
Microsoft Word, PowerPoint, Excel
Primer Design: Serial Cloner 2.6, NetPrimer, Primer3Plus, Primer-BLAST, PrimerX.
Name | Group | Language | Semester | Turn |
---|---|---|---|---|
(PAUL) Classroom practices | 331 | Catalan/Spanish | first semester | morning-mixed |
(PAUL) Classroom practices | 332 | Catalan/Spanish | first semester | morning-mixed |
(PLAB) Practical laboratories | 331 | Catalan/Spanish | first semester | afternoon |
(PLAB) Practical laboratories | 332 | Catalan/Spanish | first semester | afternoon |
(PLAB) Practical laboratories | 333 | Catalan/Spanish | first semester | afternoon |
(PLAB) Practical laboratories | 334 | Catalan/Spanish | first semester | afternoon |
(TE) Theory | 33 | Catalan/Spanish | first semester | morning-mixed |