Logo UAB
2023/2024

Applied Biocatalysis and Biotransformations

Code: 43332 ECTS Credits: 9
Degree Type Year Semester
4314579 Biological and Environmental Engineering OB 1 1

Contact

Name:
Gregorio Alvaro Campos
Email:
gregorio.alvaro@uab.cat

Teaching groups languages

You can check it through this link. To consult the language you will need to enter the CODE of the subject. Please note that this information is provisional until 30 November 2023.

Teachers

Marina Guillen Montalban

Prerequisites

Basic knowledge of:
  • Protein structure
  • Biochemistry
  • Catalysis
  • Kinetic enzyme
  • Microbial growth
  • Cell biology

Objectives and Contextualisation

The objective is to present biocatalysis and its fields of application as an alternative to classical catalysis. The knowledge of biocatalysts will be deepened as the central axis of a transformation and of the aspects of engineering of the main elements that make up biotransformation, such as the biocatalyst, the reaction medium, the bioreactor and its operation. Also the classification and study of the different biotransformations according to the biocatalyst used and different case studies. The final objective is that the student is able to define the main elements of a biotransformation and can design a biocatalytic process.


Learning Outcomes

  • CA19 (Competence) Design a biocatalytic process for its industrial application, integrating the state of the art of biocatalysis, its fields of application and the different relevant types of biotransformation.
  • CA20 (Competence) Explain the different types of biotransformation depending on the biocatalyst used, assessing the main characteristics.
  • KA02 (Knowledge) Gather the right information to determine the most suitable setup of operations for a biotechnological process.
  • KA08 (Knowledge) Distinguish between the fundamental concepts of Chemical Engineering in the different forms of reactor design and operation, including catalytic reactors and with special emphasis on reactors with immobilised biological catalysts.
  • KA08 (Knowledge) Distinguish between the fundamental concepts of Chemical Engineering in the different forms of reactor design and operation, including catalytic reactors and with special emphasis on reactors with immobilised biological catalysts.
  • KA18 (Knowledge) Distinguish the different reaction media used in biotransformations and select the appropriate reaction media.
  • KA19 (Knowledge) Distinguish the methods for immobilising biocatalysts and for characterising the immobilised biocatalyst.
  • KA20 (Knowledge) Identify multienzymatic processes.
  • SA23 (Skill) Calculate the main elements involved in biotransformation, such as the biocatalyst, the reaction medium, the bioreactor and its operation.

Content

Assignment program:

  • Topic 1 Introduction to biocatalysis
  • Topic 2 Biocatalysts
  • Topic 3 Biocatalyst engineering
  • Topic 4 Reaction medium engineering
  • Topic 5 Bioreactor operation
  • Topic 6 Biotransformations: case studies

Methodology

Directed activities:

  • Theoretical lectures: Master classes on the concepts of the subject
  • Seminars: Presentation to students of different cases of study of biotransformations and applied biocatalysis extracted from the bibliography
  • Public presentation of the work: Students will orally and publicly present a summary of the most relevant results of the work and deliver the presentation to the professor in digital format through the virtual campus

Autonomous activities:

  • Individual study: Individual study, preparation of diagrams and summaries.
  • Documentation and bibliography search: Consultation of the bibliographic and documentary sources essential for the course.
  • Realization of a work: work in groups of 2-4 students in which each group will elaborate a written work on a case study previously selected by the teacher. The work will be delivered to the teacher in paper format (printed) and in digital format through the virtual campus, in order to discuss in seminars and written presentation.
 

Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.


Activities

Title Hours ECTS Learning Outcomes
Type: Directed      
Seminars and case studies 6 0.24 CA19, CA20, KA08, KA18, KA19, KA20, SA23, CA19
Theoretical classes 49 1.96 KA08, KA18, KA19, KA20, KA08
Type: Autonomous      
Carry out work on a case study 20 0.8 CA19, CA20, KA02, KA08, KA18, KA19, KA20, SA23, CA19
Individual study 127 5.08 CA19, CA20, KA02, KA08, KA18, KA19, KA20, SA23, CA19
Search for documentation and bibliography 20 0.8 CA19, KA02, KA08, KA18, KA19, KA20, CA19

Assessment

Scheduled evaluation process and activities

Throughout the course different evaluation activities will be carried out that will lead to the final grade of the subject obtained by continuous evaluation. Specifically, the evaluable activities will be:

  • Written work on a case study that is 20% of the final grade.
  • Oral and public presentation of the written work that is 10% of the final grade
  • Attendance at all oral presentations is compulsory for all students
  • Partial exam of topics 1, 2 and 3, which represents 35% of the final grade
  • Partial examination of topics 3, 4 and 5, which represents 35% of the final grade

The written work and the oral presentation of the work are non-recoverable.

The subject is considered passed if the average of the 4 activities is 5 or higher, as long as no part is less than 4.0

If the average of the 4 activities is less than 5 or any of the parts is less than four, the suspended activities must be recovered (with less than 5)

  • If any of the following circumstances occurs, it implies a grade of Not assessable to the subject:
  • Do not perform written work
  • Do not perform the oral presentation of the work
  • Do not perform any of the exams

Programming evaluation activities

At the beginning of the subject groups will be formed to do the written work. The delivery of written work and oral presentations will be communicated through the virtual campus.

Recovery process

The student can apply for recovery whenever he has submitted to a set of activities that represent at least two thirds of the total grade of the subject. Of these, students who have on average of all the activities of the subject a grade higher than 3.5 may be presented in the recovery.

The partial exams are eliminatory, therefore, a student who has passed a partial exam will not be able to appear forthe recovery of this exam. Youwill have to compulsorily recover those partial exams where the student has obtained a grade lower than 4 regardless of the average obtained according to the calculation of the section "Process and programmed evaluation activities"

The calculation of the grade will be done in the same way as in the continuous evaluation

Procedure for review of qualifications

For each evaluation activity, a place, date and time of revision in which the student can review the activity with the teacher will be indicated. In this context, claims may be made on the activity grade, which will be evaluated by the faculty responsible for the subject. If the student does not appear in this review, this activity will not be reviewed later.

Ratings

Granting a grade of honor registration is the decision of the faculty responsible for the subject. The regulations of the UAB indicate that MH can only be granted to students who have obtained a final grade equal to or greater than 9.00. You can grant up to 5% of MH of the total number of students enrolled.

Irregularities by the student, copy and plagiarism

Without prejudice to other disciplinary measures deemed appropriate, the irregularities committed by the student that may lead to a variation of the grade of an evaluation act will be scored with a zero. Therefore, copying, plagiarism, cheating, letting copy, etc. in any of the evaluation activities will involve suspending with a zero. The evaluation activities qualified in this way and by this procedure will not be recoverable. If it is necessary to pass any of these evaluation activities to pass the subject, this subject will be suspended directly, without the opportunity to recover it in the same course. In this situation the final grade that will be reflected in the minutes will be a 2.

Evaluation of repeating students

There is no provision for a different evaluation system for repeating students.

Unique Assessment
 
This subject does not offer single assessment.

Assessment Activities

Title Weighting Hours ECTS Learning Outcomes
Oral and public presentation of the work 10% 1 0.04 CA19, CA20, KA08, KA18, KA19, KA20, SA23
Partial exam (themes 1, 2 and 3) 35% 1 0.04 CA19, CA20, KA02, KA08, KA18, KA19, KA20, SA23
Partial exam (topics 4, 5 and 6) 35% 1 0.04 CA19, CA20, KA02, KA08, KA18, KA19, KA20, SA23
Written work 20% 0 0 CA19, CA20, KA02, KA08, KA18, KA19, KA20, SA23

Bibliography

Books:

Immobilized Biocatalysts

Peter Grunwald (Ed.)

Pages: 510

Published: November 2018

ISBN 978-3-03897-318-8 (Pbk); ISBN 978-3-03897-319-5 (PDF) 

https://doi.org/10.3390/books978-3-03897-319-5

© 2018 by the authors; CC BY-NC-ND licenes

https://www.mdpi.com/books/pdfview/book/861

 

Biocatalysis for Green Chemistry and Chemical Process Development

Editor(s): Junhua (Alex) Tao, Romas Kazlauskas

First published:12 April 2011

Print ISBN:9780470437780 |Online ISBN:9781118028308 |DOI:10.1002/9781118028308

Copyright © 2011 John Wiley & Sons, Inc.

https://onlinelibrary-wiley-com.are.uab.cat/doi/book/10.1002/9781118028308

 

Carrier‐bound Immobilized Enzymes: Principles, Application and Design

Author(s): Dr. Linqiu Cao

First published:29 September 2005

Print ISBN:9783527312320 |Online ISBN:9783527607662 |DOI:10.1002/3527607668

Copyright © 2005 Wiley‐VCH Verlag GmbH & Co. KGaA

http://eds.b.ebscohost.com.are.uab.cat/eds/detail/detail?vid=0&sid=388e04a0-e014-4bc6-ba12-2daad9133ad1%40sessionmgr101&bdata=JnNpdGU9ZWRzLWxpdmU%3d#AN=161277&db=edsebk

 

 

Green Biocatalysis

Editor(s): Ramesh N. Patel

First published:13 May 2016

Print ISBN:9781118822296 |Online ISBN:9781118828083 |DOI:10.1002/9781118828083

Copyright © 2016 John Wiley & Sons, Inc.

https://cataleg.uab.cat/iii/encore/record/C__Rb2087415?lang=cat

 

 

 

Autor

Fersht, Alan, 1943-

Títol

Enzyme structure and mechanism / Alan Fersht

Edició

2nd. ed.

Publicació/producció

New York : W.H. Freeman, cop. 1985

Descripció

xxi, 475 p.; 24 cm

Matèria

Enzims

ISBN

0716716143

 

0716716151 (pbk.)

http://cataleg.uab.cat/record=b1323065~S1*cat

 

Autor

Dixon, Malcolm

Títol

Enzymes / by Malcolm Dixon and Edwin C. Webb

Edició

3rd ed.

Publicació/producció

London : Longman, 1979

Descripció

XXIII + 1116 p.; 24 cm

Matèria

Enzims

http://cataleg.uab.cat/record=b1016445~S1*cat

 

Títol

Enzyme biocatalysis : principles and applications / Andrés Illanes, editor

Publicació/producció

[Dordrecht] : Springer, 2008

Descripció

X, 391 p. : ill. ; 25 cm.

Matèria

Enzims -- Biotecnologia

 

Enzims -- Síntesi

ISBN

9781402083600

http://cataleg.uab.cat/record=b1744514~S1*cat

 

Títol

Ingeniería bioquímica/ Francesc Gòdia Casablancas y Josep López Santín (editores) ; Carles Casas Alvero ... [et al.]

Publicació/producció

Madrid : Síntesis, DL 1998

Descripció

350 p. : il.; 24 cm

Col·lecció

Ciencias químicas (Síntesis). Tecnología bioquímica y de los alimentos

Matèria

Enginyeria bioquímica

ISBN

8477386110

http://cataleg.uab.cat/record=b1425826~S1*cat

 

Autor

Bommarius, A. S.

Títol

Biocatalysis : [fundamentals and applications] / A.S.Bommarius, B.R.Riebel

Publicació/producció

Weinheim : Wiley-VCH, 2004

Descripció

XXIII, 611 p.; 24 cm

Matèria

Enzims

ISBN

3527303448

http://cataleg.uab.cat/record=b1604211~S1*cat

 

Títol

Multi-step enzyme catalysis : biotransformations and chemoenzymatic synthesis / edited by Eduardo Garcia-Junceda

Publicació/producció

Weinheim : Wiley-VCH ; Chichester : John Wiley, 2008

Descripció

241 p. ; 25 cm

Matèria

Catàlisi

 

Enzims -- Biotecnologia

ISBN

9783527319213

http://cataleg.uab.cat/record=b1747444~S1*cat

 

Títol

Immobilization of enzymes and cells / edited by Gordon F. Bickerstaff

Publicació/producció

Totowa : Humana Press, 1997

Descripció

XIV, 367 p.;23 cm

Col·lecció

Methods in biotechnology ; 1

Matèria

Enzims immobilitzats

 

Enzims -- Biotecnologia

 

Cèl·lules immobilitzades

ISBN

0896033864

http://cataleg.uab.cat/record=b1465205~S1*cat

 

Autor

Faber, Kurt

Títol

Biotransformations in organic chemistry : a textbook : with 37 figures, 238 schemes and 16 tables / Kurt Faber

Edició

5th revised and corrected ed.

Publicació/producció

Berlin: Springer-Verlag, cop. 2004

Descripció

XI, 453 p. : il; 24 cm

Matèria

Reaccions químiques

 

Química orgànica -- Reaccions

ISBN

3540200975

http://cataleg.uab.cat/record=b1615815~S1*cat

 

Autor

Grunwald, Peter

Títol

Biocatalysis : biochemical fundamentals and applications / Peter Grunwald

Publicació/producció

London : Imperial College Press, 2009

Descripció

xvi, 1035 p. : il. ; 24 cm

Matèria

Enzims -- Biotecnologia

 

Biotecnologia

ISBN

9781860947711

 

1860947719

https://cataleg.uab.cat/record=b1778458~S1*cat

 

Kourist, R. (2015), Biocatalysis in Organic Synthesis. Science of Synthesis, Vol. 1–3. Edited by Kurt Faber, Wolf-Dieter Fessner and Nicholas J. Turner.. Angew. Chem. Int. Ed., 54: 12547. doi:10.1002/anie.201508130

 

Search engines of scientific bibliography:

Scholar Google: http://scholar.google.es/advanced_scholar_search?hl=en&lr=

Scopus: http://www.scopus.com/scopus/search/form.url?display=authorLookup

Scifinder: Software disponible a la UAB

Sience Direct: http://www.sciencedirect.com/science/journals

ISI Web of Knowledge: http://www.accesowok.fecyt.es/login/ 

 

Interesting web sites:

Base de dades d’enzims BRENDA: http://www.brenda-enzymes.info/

National Center for Biotechnology Information: http://www.ncbi.nlm.nih.gov/

ExPASy (Expert Protein Analysis System) Proteomic Server: http://www.expasy.ch/


Software

No software