Degree | Type | Year | Semester |
---|---|---|---|
2501922 Nanoscience and Nanotechnology | OB | 3 | 2 |
You can check it through this link. To consult the language you will need to enter the CODE of the subject. Please note that this information is provisional until 30 November 2023.
This subject does not need any requirements.
The subject Microbiology, Immunology and Cell Culture, is taught in the 2nd semester of the 3rd year of the degree of Nanoscience and Nanotechnology in the Faculty of Sciences. This is a subject with a certain degree of specialization that is divided into three large blocks (Microbiology, Immunology and Cellular cultures) in which the students is expected to acquire some basic notions to begin with the methodologies used in the culture and manipulation of bacterial cells, in immunology laboratories and in cultures and manipulation of eukaryotic cells. That is why it is a subject with an important practical component.
Objectives of the subject:
1) To know the bacterial cell
2) To know the basic methodologies used in a Microbiology laboratory
3) To know the basic concepts of Immunology
4) To know the basic methodologies used in a Immunology laboratory
5) To know the basic equipment of a Cell Culture Laboratory
6) To know the basic methodologies used in a Cell Culture Laboratory
Theory program
Microbiology
Introduction to microbiology
Levels of organization
The bacterial cell
Techniques of observation of microorganisms
Isolation and culture techniques of microorganisms
Techniques of sterilization and conservation of microorganisms
Immunology
Basic principles of immunology: innate immunity and acquired immunity. The immune system: anatomy, cells and molecules
Components of innate immunity. Mechanisms of innate immunity. Connection between innate and acquired immunity
Components of acquired immunity. Mechanisms of acquired immunity. Immune response to pathogens
Immunopathology. Pathologies of the immune system. Pathologies that affect the immune response
Technologies related to the immune response. Immunotherapy and immunomotion
Cell cultures
Laboratory program
Microbiology
Module 1: Counting of microorganisms
Module 2: Methods of isolation of microorganisms
Module 3: Observation of microorganisms
Module 4: Identification of microorganisms
Module 5: Ubiquity and microbial diversity
Immunology
Module 6: Separation of cells from the blood by Ficoll
Module 7: Analysis of cell populations by cytometry
Module 8: Immunocytochemistry for the detection of specific markers with a monoclonal antibody
Cell cultures
Module 9: Culture of a cell line.
Module 10: Freezing / defrosting of a cell line
Module 11: Induction and detection of apoptosis in a cell line
Module 12: Detection of microtubules by immunocytochemistry. Evaluation of the phases of the cell division
Module 13: Use of nanoparticles for cell follow-up. Confocal microscopy
The subject of Microbiology, Immunology and Cell culture consists of theoretical master classes and practical classes in the laboratory.
Theoretical lectures will be made using audiovisual material prepared by the teacher, material that the students will have at their disposal in the Virtual Campus of the UAB before the sessions.
The practical classes are designed so that the students learn to use the instrumental laboratory and complement the theoretical training. The students will complete a total of 13 practical sessions with a total of about 38 hours. Students will work in groups of 2, and at the end of each practice they will have to complete a dossier with the results. These dossiers will remain in the possession of the teaching staff and will be used for the evaluation of the practical part. At the end or during the practice session the results of the different groups will be shared and will be discussed collectively.
The students will have to submit a dossier of the practices.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Laboratory classes | 38 | 1.52 | 3, 14, 9, 10, 12, 23, 22, 26, 15, 16, 19, 17, 21, 4, 24, 29, 8 |
Theoretical classes | 38 | 1.52 | 27, 7, 6, 11, 26, 19, 20, 28 |
Type: Supervised | |||
Personalized tutorials | 6 | 0.24 | 1, 3, 14, 19 |
Type: Autonomous | |||
Individual study | 105 | 4.2 | 1, 13, 27, 14, 26, 15, 16, 19, 20, 21, 28 |
Preparation of the laboratory report | 4.5 | 0.18 | 13, 3, 14, 15, 16, 19, 21 |
The subject of MICC (Microbiology, Immunology and Cell Cultures) is made up of three blocks. In order to pass the subject, a minimum score of 5 on a maximum of 10 points is required in each of the three blocks (M, I and CC). Each block consists of a part of theory and a part of practices, which represent 75% and 25% respectively, of each block. In order to pass the subject the student must obtain a mark equal to or greater than 5 in the three blocks of theory (M, I and CC) and in the three blocks of practicals (M, I and CC).
The scheduled evaluation activities are:
THEORY:
There will be an independent examination of each of the three blocks of the subject. Each of these exams will have a weight of 25% of the final grade. To overcome them, the grade must be equal to or greater than 5. Marks lower than 5 in one of the blocks will automatically imply a fail in the block and therefore the student will have to retake the subject of the block failed in a retake exam . In this retake exam, again, each block will be evaluated separately and in order to pass the subject the student will have to obtain a grade equal to or greater than 5 in each one of the blocks to retrieve.
In the examens corresponding to the theoretical part, the students must answer a minimum of 70% of the questions formulated for each of the blocks in order to be evaluated. Not answering the minimum required will imply a fail directly.
LABORATORY PRACTICES:
The practical grade will be obtained from an independent examination for each one of the blocks. In the blocks of Microbiology and Cell Culture in addition to the examination will evaluate the delivery of a dossier of practices. Attendance to practical sessions is mandatory. Students missing more than 20% of programmed sessions will be graded as "No Avaluable"
Each block will be evaluated separately and in order to pass the block the student must obtain a mark equal to or greater than 5 in each one of the examinations of the blocks. Marks lower than 5 in one of the blogs will automatically imply a fail in the blog and the student will have to retake the subject of the block failed in a retake test. Again, each block will beevaluated separately and in order to pass the subject the student will have to obtain a score equal to or greater than 5 in each one of the blocks to retake.
The note of each block will have a weight of 8.33% of the final mark and will be obtained from:
- Microbiology: exam about the work done in the laboratory. These tests will weight 100% of the grade in this block.
- Immunology: exam about the work done in the laboratory. These tests will weigh 100% of the grade in this block.
- Cell cultures: a) delivery and discussion of the different results obtained in the practises; b) exam about the work done in the laboratory; and c) Questionnaire at the end of practises. These tests will have a weight of 40%, 40% and 20%, respectively, of the grade in this block.
RECOVERY EXAM:
To be eligible for the retake process, the student should have been previously evaluated in a set of activities equaling at least two thirds of the final score of the course or module. Thus, the student will be graded as "No Avaluable" if the weighthin of all conducted evaluation activities is less than 67% of the final score
Students who have not passed one or more of the blocks of the theory exams and / or practices, or not if they have submitted, must present themselves to the retake exam.
To pass the retake exam, students must have a grade equal to or greater than 5 in each one of the blogs examined.
FINAL NOTE: The final grade of the subject will be obtained from the one of the following formula,
FINAL NOTE = [Theory (M + I + CC / 3)* 0.75] + [Practices (M + I + CC / 3) * 0.25]
Students who in any of the blocks of theory and / or practice have a score of less than 5 will have the subject failed.
Non-Valuable: Students will obtain the "Non-Valuable" qualification when the assessment activities carried out have a weighting ofless than 67% in the final grade.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Delivery of practical laboratory results | 3,5% | 0.5 | 0.02 | 1, 13, 27, 3, 25, 14, 9, 12, 2, 26, 15, 16, 18, 19, 17, 21, 5, 28 |
Laboratory exam | 18% | 3 | 0.12 | 27, 19, 20 |
Laboratory results | 3,5% | 1 | 0.04 | 3, 25, 9, 10, 12, 23, 22, 2, 15, 16, 18, 19, 21, 30, 4, 24, 29, 8 |
Theory exam | 75% | 4 | 0.16 | 27, 7, 6, 11, 19, 20, 28 |
Relevant Bibliography
Microbiology:
*Madigan, MT, JM Martinko, PV Dunlap, DP Clark. 2015. Brock Biology of Microorganisms. 14ª ed. Pearson Educación, S.A. (en paper i electrònic)
*Willey, J, LM Sherwood, CJ Woolverton. 2009. Microbiología de Prescott, Harley y Klein. 7ª ed. MacGraw-Hill-Interamericana de España. ISBN: 978-84-481-6827-8.(en paper i electrònic)
*Jennifer Louten. 2016. Essential human virology. Elsevier Ed. ISBN: 978-0-12-800947-5
*Microbiología: conceptos esenciales. Jordi Barbé García [i 39 més]. Editorial Médica Panamericana | 2019
Immunology:
*Inmunología J.Kuby, J.A. Owen, J. Punt, S.A. Strandord 7ma Ed 2014 (en paper i electrònic)
*L. Fainboim, J. Geffner. Introducción a la Inmunología Humana. 6ª edición, 2011, Editorial Panamericana. ISBN:978-9500602709 (en paper i electrònic)
*J. R. Regueiro, C. López Larrea, S. González Rodríguez, E. Martínez Naves. Inmunología: Biología y patología del sistema inmunitario, 4ª edición, 2010, Editorial Panamericana. ISBN: 978-8498350036
Cell Cultures
* A. Doyle and J.B. Griffiths Eds. Cell and Tissue Culture: Laboratory procedures in biotechnology. John Wiley & Sons Ltd. 1999. ISBN: 978-0471982555 (no hi ha cap nova edició)
* R.I. Freshney. Culture of Animal Cells: A manual of basic technique. 7th Ed. Wiley-Liss, Inc. 2010. (biblioteca 6e ed. en paper i electrònic). ISBN: 978-1-118-87365-6
* J.P. Mather and D. Barnes Eds. Animal Cell Culture Methods. Methods in Cell Biology. Academic Press. 1998.(en paper i electrònic). ISBN: 978-0124800403
No software will be used