Degree | Type | Year | Semester |
---|---|---|---|
2502445 Veterinary Medicine | OT | 5 | 0 |
There are no prerequisites for taking this subject. However, to achieve the proposed learning outcomes it is recommended to the student to have previous knowledge of Cell Biology, Genetics, Basic and Advanced Instrumental Techniques, Molecular Biology and Genetics and Recombinant DNA Technology.
It is also recommended to the students to have a basic knowledge of English in order to be able to use information sources, which are basically in this language.
The objectives of the course will focus on:
1- To provide the student with knowledge in animal transgenesis and associated technologies including the description, the types of existing transgenic animals and their function.
2- To provide the student with knowledge in the gene manipulation of cells in vitro and in vivofor therapeutic purposes and associated technologies.
THEORY PROGRAM
1- Generation of transgenic animals by pronuclear microinjection. Applications.
2- Generation of constitutive or conditional Knockout / in animals (tissue-specific and / or inducible) by Gene Targeting in embryonic stem cells. Applications.
3- Genomic Editing using ZFNs, TALENs and CRISPR / Cas9 for the generation of Knockout / in animals.
4- Clonal animals by nuclear transfer. Applications.
5- Conceptual bases of Gene Therapy.
6- In vivo gene therapy: adenoviral and adeno-associated vectors. Applications
7- Ex vivo gene therapy: retroviral and lentiviral vectors. Applications.
8- Genomic editing in gene therapy
9- Non-viral vectors. RNA therapeutics
10- Applications of gene therapy for the treatment of hereditary diseases
11- Applications of gene therapy in oncology
12- Applications of gene therapy for the treatment of high prevalence diseases.
SEMINAR PROGRAM
Students will prepare and perform an oral presentation, in front of the class, of a current research work, related to the course topics and published in international scientific journals. The preparation of this exhibition will be in groups of students and will be tutored. The oral presentation will be 10 minutes, distributed equally among the members of the group, plus 5 minutes for questions (total 15 minutes). The aim of the seminars is for students to become used to the search for scientific publications, their reading and interpretation, the critical vision of graphs, tables and results, in a guided way. On the other hand, students will also deep into current applications of transgenic animal technology and gene therapy..
CLASSROOM PRACTICES
Classroom practices will consist of the visualization of audiovisual material related to the technologies of obtaining genetically modified animals, as well as the presentation and discussion of different phenotypes of animal models and cases of gene therapy.
The course consists of theoretical classes, practical classes, and oral presentations of tutored work. The formative activities of the subject complement each other.
Theory classes:
The content of the theory program will be taught mainly by the teacher in the form of master classes with audiovisual support. The presentations used in class by the teacher will be available to students on the Virtual Campus of the subject. These expository sessions will be the most important part of the theory section. It is highly recommended for students to regularly consult the books and links in the Bibliography section of this teaching guide and in the Virtual Campus in order to consolidate and clarify, if necessary, the contents explained in class.
Classroom practices:
Classroom practices will consist of the visualization of audiovisual material related to the technologies of obtaining genetically modified animals, as well as the presentation and discussion of different phenotypes of animal models and cases of gene therapy.
Classroom practices consist on 2 sessions of 4 hours each. The dates of the different groups of practices and the classrooms will be able to consult with the sufficient advance in the Virtual Campus of the course. Attendance at practical classes is mandatory.
Oral presentations of the students (seminars):
Students will prepare and perform an oral presentation, in front of the class, of a current research work, related to the course topics and published in international scientific journals. The preparation of this exhibition will be in groups of students and will be tutored. The oral presentation will be 10 minutes, distributed equally among the members of the group, plus 5 minutes for questions (total 15 minutes). The aim of the seminars is for students to become used to the search for scientific publications, their reading and interpretation, the critical vision of graphs, tables and results, in a guided way. On the other hand, students will also deep into current applications of transgenic animal technology and gene therapy..
Tutorials:
Oral presentations of papers are tutored. In addition, at the request of students, individual tutorials will be conducted throughout the course. The aim of these sessions will be to resolve doubts, review basic concepts and guide on the sources of information consulted and on how to make a scientific presentation in public.
UAB Surveys
15 minutes of one class will be allocated for the response of the UAB institutional survey.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Practical lessons | 8 | 0.32 | 9, 2, 4, 6, 8, 1, 10, 13, 14, 12, 16 |
Theorical lessons | 16 | 0.64 | 9, 2, 3, 4, 5, 6, 7, 8, 10, 14, 11, 16 |
Type: Supervised | |||
Preparation of oral presentation | 10 | 0.4 | 9, 2, 3, 4, 5, 6, 7, 8, 1, 10, 13, 14, 12, 11, 16, 15 |
Study and bibliographic research | 28.75 | 1.15 | 9, 2, 3, 4, 5, 6, 7, 8, 1, 10, 13, 14, 12, 11, 16, 15 |
Tutorials | 10 | 0.4 | 2, 10 |
Description:
To approve the subject it will be essential to obtain a final grade of the subject equal to or greater than 5 points (out of 10) and to have attended the practices. The scheduled evaluation activities are:
A theoretical exam
It will count 90% of the final grade. It consists of a final exam, with test-type questions (true or false), referring to the subject taught in the theoretical classes and in the practical classes.
To approve the course, attendance at practical classes is mandatory.
There will be a theoretical exam for the recovery of the subject, with the same characteristics as the final theoretical exam, for students who have not approved it.
Oral presentation of the students
It will count for 10% of the final grade. The student will be evaluated individually for its oral presentation of the research work as well as the audiovisual support document that they have prepared in groups. Oral expression and the use of scientific language, aptitude and appropriate content will be valued.
A student will obtain the grade of Non-Assessable if the number of assessment activities carried out has been less than 50% of those scheduled for the subject.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Exam of practical and theorical lessons | 2 | 2 | 0.08 | 9, 2, 3, 4, 5, 6, 7, 8, 1, 10, 13, 14, 12, 11, 16, 15 |
Oral Presentation of Research Papers (Seminars) | 15 | 0.25 | 0.01 | 9, 2, 3, 4, 5, 6, 7, 8, 1, 10, 13, 14, 12, 11, 16, 15 |
Bibliografia Animals Modificats Genèticament:
- Transgenic animals. Generation and use. L.M. Houdebine. Harwood Academic Publishers 1997.
- Mouse Genetics and Transgenics. A practical approach. Edited by: I.J. Jackson and C.M. Abbott. Oxford University Press. 2000. (www.oup.co.uk/PAS)
- Gene Targeting. A practical approach. Edited by: A.L. Joyner. Oxford University Press. 2000. (www.oup.co.uk/PAS)
- Manipulating the Mouse Embryo. A laboratory manual. (3rd Edition) Edited by: Andras Nagy et al. Cold Spring Harbor Laboratory Press. 2003.
- Transgenesis Techniques. Principles and Protocols. Edited by: Alan R. Clarke. Humana Press. 2002. (2nd Edition).
- Gene Knock-out Protocols. Edited by: Martin J. Tymms and Ismail Kola. Humana Press. 2001.
- Embryonic Stem Cells. Methods and Protocols. Edited by: Kursad Turksen. Humana Press. 2002.
- Human Molecular Genetics 2. T. Strachan i A.P. Read. John Wiley & Sons, Inc., Publication. 1999.
- Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Tomas L. Saunders (Editors). Springer. 2011.
Adreces d'interés:
http://www.transtechsociety.org/
http://www.emmanet.org/
http://www.mousephenotype.org/
Bibliografia Teràpia Gènica
- Gene and Cell Therapy. Therapeutic Mechanisms and Strategies. 2nd Edition. Edited by Nancy Smyth Templeton. Marcel Dekker, Inc. 2004.
- Gene Therapy technologies, aplications and regulations. From Laboratory to Clinic. Edited by Anthony Meager. John Wiley & Sons, LTD. 1999.
- Gene Therapy Protocols. 2nd Edition. Edited by Jeffrey R. Morgan. Humana Press. 2002.
- Human Molecular Genetics 2. T. Strachan i A.P. Read. John Wiley & Sons, Inc., Publication. 1999.
- Cell Therapy. D. Garcia-Olmo, J.M. Garcia-Verdugo, J. Alemany, J.A. Gutierrez-Fuentes. McGraw-Hill Interamericana. 2008.
- Gene and Cell Therapy. Therapeutic Mechanisms and Strategies. Second edition, Revised and Expanded. N.S. Templeton. Marcel Dekker, Inc. 2004.
Not applicable