Degree | Type | Year | Semester |
---|---|---|---|
2500252 Biochemistry | OT | 4 | 0 |
The student must have passed the courses: Basic instrumental techniques, Advanced instrumental techniques and Chemistry and protein engineering.
The overall objective of the course is to learn the knowledge and skills that allow the structural analysis of biological macromolecules. The course will allow you to master the main techniques for the determination of the three-dimensional structure and visualization of biological samples by electron microscopy.
The aim is to give the maximum emphasis on the practical application of the knowledge imparted, so that students can use themselves the techniques described.
Specific objectives of the course:
1) To learn the theoretical bases of the main techniques for the analysis of the structure of macromolecules:
(2) To apply the theoretical knowledge on the structural and functional analysis of macromolecules.
THEORY PROGRAM
Unit 1. Advanced microscopy techniques.
Transmission electron microscopy: physical foundations; electron microscopes; preparation of samples; cryotechniques; electron tomography; single particle structure determination; elemental analysis; applications in Biochemistry and Molecular Biology. Scanning electron microscopy. Ion microscopy. Tunneling and atomic force microscopy: physical foundations; microscopes and imaging methods; preparation of samples; force spectroscopy; nanotribology; applications in Biochemistry and Molecular Biology.
Unit 2. Applications of synchrotron radiation
Introduction: what is a synchrotron? Basis and Applications in biomedicine: reflection , scattering, absorption and fluorescence of X-ray , microscopy, of X-Ray and IR
Unit 3. X-ray crystallography and applications.
Theoretical foundations of the determination of the three-dimensional structure of macromolecules using X-ray diffraction and crystallography; methods of crystallization; properties of crystals; obtaining and processing of diffraction data; Electron density maps; reconstruction of the model; validation of the model quality; tools to visualize and identify functional regions in 3D macromolecules.
Unit 4.Bioinformatics Tools applied to the structural analysis of macromolecules.
Introduction to the Unix operating system. Databases. Structures comparison methods. Calculation of structural and biophysical parameters. Study of structural complexes. Identification of functional domains. Graphical applications for analysis and visualization of macromolecules. Modeling of macromolecules and applications for drug design.
Problems
The resolution of practical cases that will facilitate the consolidation of the theoretical concepts will be performed. Most of the problems’ teaching will be held in the computer room.
Tutorials
Several tutoring sessions can be performed during the semester. These sessions aim to answer questions and review concepts.
LABORATORY PRACTICES
Three practical sessions will be held:
1st session:Practices of microscopy at the UAB microscopy service.
2nd session: Practice in the classroom's computer SID.
3rd session: Guided tour of the ALBA Synchrotron Light laboratory. Seminar by Dr. Fernando Gil and explanation of the operation stations of X-ray microscopy, BL-09; non-crystalline diffraction, BL-11, and Macromolecule crystallography, BL-13.
Theoretical master classes
The teacher will explain the contents of the program with the support of audiovisual material that will be available for students at the Moodle/Virtual Campus section. This support material will be written in English, Catalan or Spanish.
Optionally, seminars by specialists in the field will be held.
Problem cases
Throughout the course you will attend 8 hours of problems’ teaching. Classes will include sessions at the computer room.
Practices
There will be guided tours to large installations with specialized equipment. The Protocol of practices will be available at the Virtual Campus before the practice session. Practices will also include sessions at the computer room.
Students must attend the practice session with the Protocol (available at the Virtual Campus) printed and read beforehand and bring a notebook to write down observations and data.
Practices, as well as its evaluation, will be carried out individually or in groups of two people. Attendance at practical sessions is mandatory, except in cases where there is a justified reason to prove the student absence.
Tutorials
Several tutorial sessions can be held during the semester. The aim of these sessions is to answer questions and review concepts with a high level of difficulty.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Theoretical lectures | 30 | 1.2 | 3, 4, 6, 7, 9, 8 |
Type: Supervised | |||
Practicum | 9 | 0.36 | 2, 4, 5, 6, 11, 14 |
Problems | 10 | 0.4 | 2, 3, 4, 5, 6, 7, 8, 11, 14 |
Type: Autonomous | |||
Autonomous work | 52.5 | 2.1 | 2, 5, 15, 6, 7, 9, 8, 11, 13, 14 |
solving of practical cases | 41 | 1.64 | 2, 5, 15, 6, 7, 9, 10, 11, 13, 14 |
Description
The qualification will be based on the following elements:
1 - Final test of theoretical content: a maximum of 7 points
2 – Problems’ reports: maximum 1.5 points
3 - Participation in practices: maximum 1.5 points
The content of the course will be evaluated in two partial exams.
The proportional weight in the final mark for each of the issues will be proportional to the number of hours taught by each teacher.
The course will be overcome when the final mark is equal to or greater than 50 for a maximum of 100.
Other considerations
Students who cannot attend an individual evaluation test due to a justified cause must provide an official documentation to the Coordinator of the course and shall be entitled to perform the corresponding test in a different date.
To be eligible for the retake process, the student should have been previously evaluated in a set of activities equaling at least two thirds of the final score of the course or module. Thus, the student will be graded as "No Avaluable" if the weighthin of all conducted evaluation activities is less than 67% of the final score
Rules for improving your mark:
It is possible to improve the note of the midterms exam on the occasion of the Recovery Examination. The second note obtained will be considered asfinal ifthis one is higher than the one obtained in the first test.
When the obtained note at the second chance is less than 1 point or more than the first note obtained, the final note considered will be the average of the two notes.
The student will have 10 minutes at the start of the test to decide whether or not to perform the test.
For the maximum awardof honours qualification priority will be given to qualifications obtained in midterms’ exam.
Calculation of the final mark:
Final mark = 0.70 * Theory + 0.15 * Problems + 0.15 * Practices
To pass the course the final mark must be≥ 5
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Evaluation 1st+ 2nd part Theory Exam | 70% | 5.25 | 0.21 | 2, 3, 4, 5, 15, 6, 7, 9, 10, 8, 11, 13, 14 |
Practicum evaluation | 15% | 1 | 0.04 | 2, 5, 9, 10, 11, 14 |
Problems Evaluation | 15% | 1.25 | 0.05 | 1, 2, 5, 7, 10, 11, 12, 14 |
Molecular Biology of Assemblies and Machines.A. C. Steven et al. (2016) Garland Science.
Biophysical Chemistry D. Klostermeier & MG Rudolph (2017) CRC Press
Proteins. Structures and Molecular Properties. T.E. Creighton, (1993) 2ed Freeman W.H.and co
Introduction to Biophysical Methods for Protein and Nucleic Acid ResearchGläsel and Deutscher (1995) Academic Press
Crystal Structure Analysis for Chemists and Biologists.J.P. Glusker, M. Lewis and M. Rossi (1994) VCH Publishers, Inc.
Llibres electrònics de lliure accés a la biblioteca de la UAB:
Integrative Structural Biology with Hybrid Methods Advances in Experimental Medicine and Biology. Vol. 1105. Haruki Nakamura; Gerard Kleywegt; Stephen K. Burley and John L. Markley. Springer. Cohen et al. editors. 2018
Web links
http://www-structmed.cimr.cam.ac.uk/course.html
http://www.doitpoms.ac.uk/tlplib/crystallography3/index.php
UCSF Chimera; CCP4i2; Coot, Phenix; Modeller, Autodockv4.