Degree | Type | Year | Semester |
---|---|---|---|
2500252 Biochemistry | OB | 1 | 1 |
There are no prerequisites for this course. The student is advised to refresh the chemistry and biology knowledge acquired during the "batxillerat".
The general objective of this course is to familiarise the student with the basic techniques used in the biochemistry/molecular biology laboratory. This is a classroom-based course centred in theoretical aspects, which will be worked out in the lab in the Laboratori integrat I and II, scheduled later during the academic year.
Safety, good practices, laboratory notebook, use of materials and reagents, quantitative transfer of liquids, pipette use, statistical analysis, computer tools. Buffers, electrodes, biosensors. Sample preparation techniques.
Basic principles and instrumentation: Beer-Lambert law. UV, visible and fluorescence spectroscopy.
Applications, protein and nucleic acid quantification.
Basic principles.
Instrumentation. Types of centrifuges depending on their speed: low, high and ultracentrifugation. Rotor types.
Applications: fractional centrifugation for subcellular fractionation, gradient density centrifugation for the separation of blood coponents.
Analytical centrifugation.
Basic principles. Types and materials: Planaar and column chromatography. Gel filtration, ionic exchange, affinity. HPLC.
Basic types and applications.
Basic principles.
Electrophoretic methods and instrumentation. Supports: paper, agarose and polyacrylamide.
Applications: nucleic acid separation in agarose gels and polyacrilamide gel electrophoresis (PAGE) for protein separation. PAGE types.
Isolation and characterisation of DNA, restriction digestion of DNA. Nucleic acid amplification: the polymerase chain reaction (PCR). Basic principles of PCR and applications.
Antibody production, immunoelectrophoresis, Western blot, enzyme-linked immunosorbent assay (ELISA), immunofluorescence, fluorescent activated cell sorting (FACS), LFA.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Lectures | 25 | 1 | 1, 13, 12, 4, 6, 5, 8, 7, 10, 9, 3, 11, 14 |
Type: Supervised | |||
Tutorial hours | 8 | 0.32 | 2, 4, 6, 5, 8, 7, 10, 9, 3, 11, 14 |
Type: Autonomous | |||
Autonomous learning: MOODLE activities | 20 | 0.8 | 1, 13, 12, 2, 8, 7, 3, 11, 14 |
Self-study | 10 | 0.4 | 4, 6, 5, 7, 10, 9, 11, 14 |
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
MOODLE activities | 30% | 8 | 0.32 | 1, 13, 12, 2, 8, 3, 11, 14 |
Problem solving test | 35% | 2 | 0.08 | 4, 6, 5, 7, 10, 9, 11, 14 |
Theoretical concepts test | 35% | 2 | 0.08 | 4, 6, 5, 7, 10, 9, 11, 14 |
• Biochemistry Laboratory: Modern Theory and Techniques, 2nd Edition, 2012. Rodney Boyer. Ed. Pearson. ISBN: 9780136043027.
• Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology, 8th Edition, 2018. Andreas Hofmann and Samuel Clokie. Ed. Cambridge University Press. ISBN: 9781316614761.
• Técnicas instrumentales de análisis en Bioquímica. Juan Manuel García Segura. 1999. Ed. Síntesis. ISBN: 8477384290.
• Calculations for Molecular Biology and Biotechnology. Frank Stephenson. 3rd Edition. 2016. Ed. Elsevier. ISBN: 9780128022115. https://www-sciencedirect-com.are.uab.cat/science/book/9780128022115
• Biochemical Calculations: How to Solve Mathematical Problems in General Biochemistry, 2nd Edition, 1976. Irwin Segel. Ed. Wiley. ISBN: 978-0-471-77421-1
• Fundamentals of Biochemical Calculations. Second Edition. 2008. Krish Moorthy. Ed. CRC Press. ISBN: 9780429142185.
• Biomedical Science Practice. Experimental and Professional Skills (Fundamentals of Biomedical Science). Glencross, H. Ahmed, N. Wang, Q. 1ª Ed. 2010. ISBN 9780199533299. Oxford University Press.
• Practical Skills in Biomolecular Science 5th edn, 5th Edition. Weyers & Jones (2017) ISBN-13: 9781292100739. Pearson Eds.
• Recursos web indicats pel professor a través de MOODLE.
No special programs are needed. Only a computer with web access, Excel/Googlesheets and PowerPoint/GoogleSlides or similar and a standard PDF viewer.