Degree | Type | Year | Semester |
---|---|---|---|
4313782 Cytogenetics and Reproductive Biology | OT | 0 | 2 |
The same prerequisites for admission to the Master.
The module "Laboratori integrat de citogenètica" is a compulsory practical module of the Citogenetics specialty. The main objective is to provide students with the basic tools to apply cytogenetic techniques used in genetic diagnostic laboratories and in research laboratories.
The training objectives of this module are:
1- Acquire the ability to work in sterile conditions to establish a cell culture and obtain cells at different mitotic stages.
2- Identify different types of cell culture contamination.
3- Obtain histological tissue sections and apply different stains.
4- Learn to use different types of microscopes: clear field, fluorescence and confocal laser scanning.
5- Identify human chromosomes according to the pattern of G-bands and make the karyotype. Detect the alterations of this pattern of bands.
6- Detect proteins and DNA sequences using immunocytofluorescence and fluorescence in situ hybridization techniques (FISH), respectively.
7- Identify genes affected in a region of the genome, search for their sequence and produce a fluorochrome labeled DNA probe to apply FISH techniques.
1. Update in histological techniques
2. Cell culture, fluorescence in situ hybridization (FISH) and immunocyte fluorescence
3. Application of bioinformatic tools
4. Confocal laser scanning microscopy
5. Chromosome identification: karyotype
Note: Unless the requirements enforced by the health authorities demand a prioritization or reduction of these contents.
The module "Integrated Cytogenetics Laboratory" is basically practical, distributed in 5 blocks. The practices will be done in the laboratory, in the bioinformatics classroom and the Microscopy Service of the UAB.
Note: The proposed teaching methodology may experience some modifications depending on the restrictions to face-to-face activities enforced by health authorities.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Bioinformatic tools application | 4 | 0.16 | 4, 3, 9, 14, 7, 10, 16 |
Cell culture, fluorescence in situ hybridization (FISH) and immunocytofluorescence | 31 | 1.24 | 4, 3, 2, 5, 13, 8, 9, 14, 16 |
Chromosome identification: karyotype | 5 | 0.2 | 3, 13, 11, 14, 16 |
Confocal laser scanning microscopy | 10 | 0.4 | 3, 13, 9, 12 |
Update in histological tecniques | 20 | 0.8 | 4, 3, 1, 13, 9 |
Type: Supervised | |||
Personalized tutoring | 30 | 1.2 | 11, 14, 6, 7 |
Photographic composition preparation | 5 | 0.2 | 14, 7, 16 |
Preparation of the practices reports | 15 | 0.6 | 3, 13, 17, 11, 14, 6, 7, 15 |
Problems and pratical cases preparation | 10 | 0.4 | 4, 3, 13, 9, 17, 11, 14, 6, 7, 15, 16 |
Type: Autonomous | |||
Report preparation of the practices results | 20 | 0.8 | 13, 9, 17, 11, 6, 7, 15, 16 |
Resolution of practical cases or problems | 12 | 0.48 | 4, 3, 13, 8, 9, 17, 11, 14, 6, 7, 15, 16 |
Study | 63 | 2.52 | 17, 11, 14, 6, 7, 15, 16 |
In order to pass the module, it will be necessary to obtain a global qualification equal to or greater than 5 out of a maximum of 10.
Attendance at practical sessions is mandatory. The students will obtain the "No Avaluable" qualification when the absence exceeds 20% of the programmed sessions.
The final qualification results from the weighted sum of the mark obtained in each evaluation block. The weight of each evaluation block is proportional to the time spent in the sessions programmed to perform these activities. In each block, attitude and active participation represent 10% of the qualification.
The competences of this module will be evaluated as follows:
1. Update in histological techniques (29% of the module's qualification):
2. Cell culture, fluorescence in situ hybridization and immunocytofluorescence (44% of the module's qualification):
3. Application of bioinformatic tools (6% of the module's qualification):
4. Confocal Laser Scanning Microscopy (14% of the module's qualification):
5. Chromosome identification: karyotype (7% of the module's note):
Recovery/ Retake process
The "Integrated Cytogenetics Laboratory" module, being eminently practical, does not allow the existence of recovery tests, unless, the responsible teacher of the block, with the approval of the module coordinator, can schedule a recovery.
To be eligible for the retake process, the student should have been previously evaluated in a set of activities equalling at least two thirds of the final score of the course. Thus, the student will be graded as "No Avaluable" if the weighting of all conducted evaluation activities is less than 67% of the final score.
“No Avaluable” qualification
Students will get the "No Avaluable" qualification when:
Note: Student’s assessment may experience some modifications depending on the restrictions to face-to-face activities enforced by health authorities.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Application of bioinformatic tools | 6% | 0 | 0 | 4, 9, 14, 6, 7, 10, 16 |
Cell culture, fluorescence in situ hybridization and immunocytofluorescence | 44% | 0 | 0 | 4, 3, 2, 5, 13, 8, 9, 17, 11, 14, 6, 7, 10, 15, 16 |
Chromosome identification: karyotype | 7% | 0 | 0 | 3, 11, 14, 6, 7, 16 |
Confocal Laser Scanning Microscopy | 14% | 0 | 0 | 4, 3, 13, 11, 14, 6, 7, 12, 16 |
Update in histological techniques | 29% | 0 | 0 | 4, 1, 13, 14, 7, 16 |
Books
Webs
The specific bibliography corresponding to the different contents of the module may be requested to the responsible teachers of each block.