Molecular Biology and Biotechnology of Plants
Code: 100963 ECTS Credits: 6| Degree | Type | Year | Semester |
|---|---|---|---|
| 2500253 Biotechnology | OT | 4 | 0 |
Contact
- Name:
- David Caparros Ruíz
- Email:
- David.Caparros@uab.cat
Use of Languages
- Principal working language:
- catalan (cat)
- Some groups entirely in English:
- No
- Some groups entirely in Catalan:
- Yes
- Some groups entirely in Spanish:
- No
Teachers
- Jordi Moreno Romero
- Maria del Mar Marquès Bueno
Prerequisites
There are no mandatory prerequisites.
Objectives and Contextualisation
The general goal of this subject is to provide the required knowledge to understand the molecular bases of plant biology, as well as the techniques and basic aspects of plant biotechnology, with important social implications as well as the use of transgenic plants or Genetically Modified Organisms (GMOs).
At the end of this subject, students should be able to have their own criteria on issues of plant biotechnology with social repercussion, based on contrasting knowledge. The topics that will be addressed in the subject can be seen in the content section.
Competences
- Apply the principal techniques for the use of biological systems: recombinant DNA and cloning, cell cultures, manipulation of viruses, bacteria and animal and plant cells, immunological techniques, microscopy techniques, recombinant proteins and methods of separation and characterisation of biomolecules.
- Comply with ethical principles and legislation in the manipulation of biological systems.
- Describe the molecular, cellular and physiological bases of the organisation, functioning and integration of living organisms in the framework of their application to biotechnological processes.
- Interpret experimental results and identify consistent and inconsistent elements.
- Learn new knowledge and techniques autonomously.
- Read specialised texts both in English and one’s own language.
- Reason in a critical manner
- Search for and manage information from various sources.
- Work individually and in teams
Learning Outcomes
- Describe European Union legislation on plant biotechnology.
- Describe the characteristics and organisation of the genome of the different organelles in plant cells, and their coordinated expression.
- Describe the different methods for obtaining transgenic plants.
- Describe the processes involved in the interaction and communication of plants with their environment and their adaptation to situations of environmental stress.
- Explain and evaluate the application of transgenic plants to plant improvement.
- Explain the molecular bases of the principal biological processes in plants, including development and immunological responses.
- Interpret experimental results and identify consistent and inconsistent elements.
- Learn new knowledge and techniques autonomously.
- Perform a diagnosis in plant biotechnology. Identify plant varieties by analysing genetic markers.
- Read specialised texts both in English and one’s own language.
- Reason in a critical manner
- Search for and manage information from various sources.
- Work individually and in teams
Content
The subject will be organized in two sections: a first section in which theoretical knowledge will be imparted and a second one in which students will learn by the resolution of different scientific problems.
In relation to the first section of the subject, students will learn the following concepts and knowledge:
-Structure of a plant gene: from the transcription to the functional protein.
-Plant transformation: via Agrobacterium tumefaciens, via bio-ballistics, via chemical mutations.
-Generation of transgenic plants by over-expression of a gene of interest (with the promoter 35S), or repression with the technique of RNAi.
-Gene-editing using the CRISPR-Cas technique.
-Mutants: what they are, why they are used for, how are they generated, the importance of mutant collections.
-Arabidopsis thaliana as a model organism and its comparison with other plants.
-Use of bioinformatics platforms for molecular biology studies.
-omics techniques for the study of the regulation of gene expression.
In relation to the second section of the subject, students will have to solve proposed problems in the field of plant molecular biology.
The theoretical content will be evaluated with an exam that will be the main body of the final mark.
*Unless the requirements enforced by the health authorities demand a prioritization or reduction of these contents.
Methodology
Methodology
The formative activities will be: theory, seminars and laboratory.
Theory
Teachers will explain the contents of the subjects using material available on the Internet. These lectures will be the first part of the subject. The knowledge of some parts of the subjects will have to be also developed by the students, by means of autonomous learning. To facilitate this task, we will provide information on textbooks, web pages, scientific articles related to the topic ...
Seminars
The seminars will be given by the students, individually or in groups, depending on the number of students and the availability of time.
The students will have to present in 10 minutes a current problem of plant molecular biology and emphasize the objectives for its resolution.
The topics that will cover the seminars will be decided at the beginning of the course. The aim of these sessions is to deepen the knowledge given in the lectures, as well as to have discussion on topics of special interest for the students.
The seminars will be evaluated, with an impact on the final mark.
Laboratory
The practical classes of laboratory will consist of 3 sessions of 4 hours each one. The protocols used will be provided at the beginning of the academic year. During these sessions, experimental skills in some of the basic issues of plant biotechnology will be acquired.
The practices will be evaluated, having an impact on the final mark.
*The proposed teaching methodology may experience some modifications depending on the restrictions to face-to-face activities enforced by health authorities.
Activities
| Title | Hours | ECTS | Learning Outcomes |
|---|---|---|---|
| Type: Directed | |||
| laboratory training | 12 | 0.48 | 7, 11, 9, 13 |
| lectures | 28 | 1.12 | 12, 3, 4, 1, 2, 5, 6, 10, 11, 9 |
| seminars | 12 | 0.48 | 12, 1, 7, 10, 11, 13 |
| Type: Supervised | |||
| Examination (seminars and theory) | 7 | 0.28 | 12, 3, 4, 2, 5, 6, 10, 11, 13 |
| tutoring | 5 | 0.2 | 12, 3, 4, 2, 5, 6, 7, 10, 11, 9 |
| Type: Autonomous | |||
| elaboration of practical report | 5 | 0.2 | 12, 7, 11, 9, 13 |
| seminar elaboration | 5 | 0.2 | 12, 10, 11, 13 |
| studies | 69 | 2.76 | 8, 12, 3, 4, 2, 5, 6, 10, 11, 13 |
Assessment
Laboratory practices, seminars and the acquisition of knowledge corresponding to the subject explained and worked in theoretical classes will be evaluated separately.
Attendance to the laboratory sessions is MANDATORY. Failure to meet this requirement will imply that the student loses the right to be evaluated in the rest of the evaluation parts. Students missing more than 20% of programmed sessions will be graded as "No Avaluable".
Once the practices have been approved, it will not be necessary to do them again, even if the student may enroll in this subject again. The following aspects will be evaluated: 1) attitude and participation; 2) experimental results obtained; 3) final report. The report, with a length of between 5 and 10 pages, will consist of a presentation of the results obtained and in the elaboration and critical discussion of these results. The maximum mark for laboratory practices is 1.5 points.
The seminars involve an oral presentation (3 minutes talk) on the corresponding subject, in the presence of the whole class. The maximum possible mark for the seminars is 1 point. Elaboration and exposition of a research project is 1 point.
The acquisition of knowledge corresponding to the subject explained in the theoretical classes will be evaluated by means of:
A test of concepts in the middle of the teaching period, which will be qualified with a maximum mark of 1.5 points.
An exam at the end of the teaching period, which will be qualified with a maximum mark of 5.0 points.
The final mark of the subject will be obtained by the sum of all the marks obtained in the different parts (laboratory, seminars and theory). Approving the subject will involve obtaining a minimum of 5.0 total points. In addition, to approve the subject, the mark of the theory exam may not be under 1.7 points, with a maximum of 5.0 points. Otherwise, the subject will be suspended, although the sum ofthe different notes gives a score of 5.0 or more.
Students who have not passed the subject will have the option to be re-evaluated of the theoretical part. To be eligible for the retake process, the student should have been previously evaluated in a set of activities equaling at least two thirds of the final score of the course. Thus, the student will be graded as "No Avaluable" if the weighthin of all conducted evaluation activities is less than 67% of the final score"
The students who wish to improve their mark will have also access to the retake process. In this case it is understood that they renounce the previously obtained theory mark.
*Student’s assessment may experience some modifications depending on the restrictions to face-to-face activities enforced by health authorities.
Assessment Activities
| Title | Weighting | Hours | ECTS | Learning Outcomes |
|---|---|---|---|---|
| design research project and oral presentation | 10 | 1.5 | 0.06 | 12, 7, 10, 11 |
| final exam | 50 | 3 | 0.12 | 8, 12, 3, 4, 2, 5, 6, 10, 11 |
| laboratory training | 15 | 0 | 0 | 12, 1, 5, 7, 11, 9, 13 |
| seminars | 10 | 1.5 | 0.06 | 12, 5, 7, 10, 11, 13 |
| test of concepts | 15 | 1 | 0.04 | 4, 5, 11 |
Bibliography
- Biochemistry and Molecular Biology of Plants (Buchanan, Gruissem and Jones) ASPP.
- Biology of Plants (Raven, Evert, and Eichhorn) Worth publishers,Inc.
- Plant Physiology (Salisbury and Ross) Wadsworth Publishing Company
- Plants, Genes, and Agriculture (Chrispeels and Sadava). Jones and Bartlett Publishers
- Fundamentos de Fisiología Vegetal. Joaquín Azcón-Bieto y Manuel Talón (2000). McGraw-Hill Interamericana y Edicions de la Universitat de Barcelona.
- Huellas de DNA en genomas de plantas (Teoría y protocolos de laboratorio). Ernestina Valadez Moctezuma y Günter Kahl (2000). Mundi-Prensa México.
- Biotecnología Vegetal. Manuel Serrano García y M. Teresa Piñol Serra (1991). Colección Ciencias de la Vida. Editorial Síntesis. Madrid.
- ARTÍCULOS Y REVISIONES DE DIFERENTES REVISTAS CIENTÍCAS DEL CAMPO. PRÁCTICAMENTE LA TOTALIDAD DE ESTE TIPO DE BIBLIOGRAFÍA ES EN INGLÉS.
Student will find all the required theory information through the online tools that are available at the University.