Degree | Type | Year | Semester |
---|---|---|---|
2500253 Biotechnology | OT | 4 | 0 |
Fluent knowledge (spoken and written) of Catalan and / or Spanish
To achieve the objectives of the course it is recommended to have some solid basic knowledge in:
Biochemistry
Enzymatic kinetics
Microbial kinetics
Cell biology
Enzymology
Relationship structure / function of proteins
Bioreactors
Organic chemistry
Description and objectives:
The immobilization of biocatalysts can be understood as a whole series of techniques that allow to have the biocatalyst located in a limited space, while maintaining its activity. A biocatalyst is an enzyme, a cell or a cellular organelle. In the case of cells, the maintenance of its activity is usually linked to its viability. The process of biocatalyst immobilization opens up a whole range of possibilities in its use, mainly due to the fact that by means of immobilization it is passed from having a microscopic biocatalyst, normally dissolved or dispersed in a liquid medium, to a macroscopic biocatalyst, usually attached to a solid matrix. These possibilities range include: the development of continuous bioprocesses in a much more intensive and productive way, to obtain biosensors or bioxips and the improvement of purification processes.
The first aim of the subject is to present the different techniques with which the process of immobilization of biocatalysts can be addressed, with particular emphasis on how to alter the design of the immobilized biocatalyst for a desired application. In a second block, the aspects associated with the physical processes derived from having the biocatalysts in a solid matrix are analyzed in greater detail. Finally, a series of specific applications are analyzed, which serve to see the impact of immobilization in the whole of a particular application in Biotechnology.
It is basically intended to establish the relationship between the nature of the biocatalyter employed, the different immobilization methods available and the final application that is intended to be developed, analyzing different alternatives and modifications in the design of the particles and the final system to be developed.
Program:
Topic 1. Introduction
Topic 2. Immobilization of biocatalysts: definition and classification
Topic 3. Immobilization of biocatalysts. Advantages
Topic 4. Immobilization of biocatalysts. Disadvantages
Topic 5. Basic steps during the immobilization of Biocatalysts
Topic 6. Mass transfer limitations in immobilized biocatalysts: External and internal
Topic 7. Monitoring of the immobilization process
Topic 8. Immobilization by adsorption
Topic 9. Immobilization by covalent bond
Topic 10. Immobilization by crosslinking. Self-immobilization
Topic 11. Immobilization by entrapment
Topic 12. Immobilization by encapsulation in membranes
Directed activities:
• Theoretical classes: Master classes on the concepts of the syllabus
• Laboratory practices: Students performed laboratory practices in which they will gain practical experience in the immobilization of biocatalysts.
• Public presentation of works: Students will present orally (10-20 minutes) and publicly a summary of the most relevant results of the work on immobilization techniques and will deliver the presentation to the professor in digital format through the virtual campus. Both the documentation of the work and oral presentations are part of the content of the subject and therefore are subject of examination
Autonomous activities:
• Student study: Individual study and preparation of diagrams and summaries
• Preparation of practical reports: group work of 2-4 students in which each group will prepare a report with the results obtained in the laboratory practices and will deliver the report to the professor in paper format (printed) and in digital format through the campus virtual
• Writing of papers: group work of 2-4 students in which each group will write two written works. A work on immobilization techniques and another on biocatalysts. Both papers must be submitted to the teacher in paper format (printed) and in digital format through the virtual campus. Both the documentation of the work and oral presentations are part of the content of the subject and therefore are subject of examination
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Laboratory practices | 15 | 0.6 | 1, 10, 11 |
Public presentation of works | 4 | 0.16 | 7, 11 |
Theoretical classes | 36 | 1.44 | 2, 4, 3, 5, 6, 9 |
Type: Autonomous | |||
Drafting of works | 34 | 1.36 | 9, 8, 10, 11 |
Elaboration report of practices | 9 | 0.36 | 9, 8, 10, 11 |
Study of the student | 49 | 1.96 | 9, 10, 11 |
Scheduled evaluation process and activities
Throughout the course different evaluation activities will be carried out that will give rise to the final grade of the subject obtained by continuous evaluation. Specifically, the evaluation activities will be:
The laboratory practices and the oral presentation of the work of immobilization techniques are non-recoverable.
The subject is considered to be passed if the average of the 5 evaluation activities is 5 or higher as long as no activity has a grade lower than 4.
If any of the following circumstances occurs, it implies a grade of Not assessable to the subject:
No note is saved for the next course.
Programming evaluation activities
At the beginning of the subject groups will be formed to do the lab work and practices. The delivery of written papers and oral presentations will be communicated through the virtual campus.
Recovery process
The written work is eliminatory, therefore, a student who has passed (grade equal to or greater than 4) the work can notbe submitted to the recovery of these works. There will be compulsory recovery of those works where the student has obtained a grade lower than 4 regardless of the average obtained according to the calculation of the section "Process and programmed evaluation activities"
The student can apply for recovery whenever he has submitted to a set of activities that represent at least two thirds of the total grade of the subject. Of these, students who have, on average of all the activities of the subject, a grade higher than 3.5 may be presented in the recovery.
The exam of synthesis is eliminatory, therefore, a student who has passed (grade equal or superior to 4) the exam can not be presented to the recovery of the exam. You will have to compulsorily recover this exam if the student has obtained a grade lower than 4 regardless of the average obtained according to the calculation of the section "Process and programmed evaluation activities"
The calculation of the grade will be done in the same way as in the continuous evaluation.
Procedure for review of qualifications
For each evaluation activity, a place, date and time of revision in which the student can review the activity with the teacher will be indicated. In this context, claims may be made on the activity grade, which will be evaluated by the faculty responsible for the subject. If the student does not appear in this review, this activity will not be reviewed later.
Ratings
Granting a grade of honor registration is the decision of the faculty responsible for the subject. The regulations of the UAB indicate that MH can only be granted to students who have obtained a final grade equal to or greater than 9.00. You can grant up to 5% of MH of the total number of students enrolled.
It will be considered as Not Evaluated that student who, not having passed the subject by continuous evaluation, does not show up for the recovery.
Irregularities by the student, copy and plagiarism
Without prejudice to other disciplinary measures deemed appropriate, the irregularities committed by the student that may lead to a variation of the grade of an evaluation act will be scored with a zero. Therefore, copying, plagiarism, cheating, letting copy, etc. in any of the evaluation activities will involve suspending with a zero. The evaluation activities qualified in this way and by this procedure will not be recoverable. If it is necessary to pass any of these evaluation activities to pass the subject, this subject will be suspended directly, without the opportunity to recover it in the same course. In this situation the final grade that will be reflected in the minutes will be a 2.
Evaluation of repeating students
There is no provision for a different evaluation system for repeating students.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Laboratory practices | 25% | 0 | 0 | 1, 9, 11 |
Oral presentations of the work of immobilization techniques | 5% | 0 | 0 | 2, 3, 7, 8, 10 |
Synthesis test | 40% | 3 | 0.12 | 2, 4, 3, 5, 6, 10 |
Written work on biocatalysts | 15% | 0 | 0 | 4, 9, 8, 10, 11 |
Written work on immobilization techniques for biocatalysts | 15% | 0 | 0 | 9, 8, 10, 11 |
Books:
Autor |
|
Títol |
Enzyme structure and mechanism / Alan Fersht |
Edició |
2nd. ed. |
Publicació/producció |
New York : W.H. Freeman, cop. 1985 |
Descripció |
xxi, 475 p.; 24 cm |
Matèria |
|
ISBN |
0716716143 |
0716716151 (pbk.) |
http://cataleg.uab.cat/record=b1323065~S1*cat
Autor |
|
Títol |
Enzymes / by Malcolm Dixon and Edwin C. Webb |
Edició |
3rd ed. |
Publicació/producció |
London : Longman, 1979 |
Descripció |
XXIII + 1116 p.; 24 cm |
Matèria |
http://cataleg.uab.cat/record=b1016445~S1*cat
Autor |
|
Títol |
Carrier-bound immobilized enzymes : principles, applications and design / Linqiu Cao |
Publicació/producció |
Weinheim : Wiley-VCH, cop. 2005 |
Descripció |
XV, 563 p. : il.; 24 cm |
Matèria |
|
ISBN |
3527312323 |
9783527312320 |
http://cataleg.uab.cat/record=b1736123~S1*cat
Títol |
Enzyme biocatalysis : principles and applications / Andrés Illanes, editor |
Publicació/producció |
[Dordrecht] : Springer, 2008 |
Descripció |
X, 391 p. : ill. ; 25 cm. |
Matèria |
|
ISBN |
9781402083600 |
http://cataleg.uab.cat/record=b1744514~S1*cat
Títol |
Ingeniería bioquímica / Francesc Gòdia Casablancas y Josep López Santín (editores) ; Carles Casas Alvero ... [et al.] |
Publicació/producció |
Madrid : Síntesis, DL 1998 |
Descripció |
350 p. : il.; 24 cm |
Col·lecció |
Ciencias químicas (Síntesis). Tecnología bioquímica y de los alimentos |
Matèria |
|
ISBN |
8477386110 |
http://cataleg.uab.cat/record=b1425826~S1*cat
Autor |
|
Títol |
Biocatalysis : [fundamentals and applications] / A.S.Bommarius, B.R.Riebel |
Publicació/producció |
Weinheim : Wiley-VCH, 2004 |
Descripció |
XXIII, 611 p.; 24 cm |
Matèria |
|
ISBN |
3527303448 |
http://cataleg.uab.cat/record=b1604211~S1*cat
Títol |
Immobilized enzymes and cells / edited by Klaus Mosbach |
Publicació/producció |
Orlando (Fla.) : Academic Press, 1987 |
Descripció |
vol. : il., gràf.; 24 cm |
Col·lecció |
|
Matèria |
|
ISBN |
0121820351 (vol. B) |
012182036X (vol. C) |
|
0121820378 (vol. D) |
http://cataleg.uab.cat/record=b1825278~S1*cat
Títol |
Immobilization of enzymes and cells / edited by Gordon F. Bickerstaff |
Publicació/producció |
Totowa : Humana Press, 1997 |
Descripció |
XIV, 367 p.; 23 cm |
Col·lecció |
|
Matèria |
|
ISBN |
0896033864 |
http://cataleg.uab.cat/record=b1465205~S1*cat
Títol |
Protein stability and folding : theory and practice / edited by Bret A. Shirley |
Publicació/producció |
Totowa (N.J.) : Humana Press, cop. 1997 |
Descripció |
X, 377 p. : gràf. , 23 cm |
Col·lecció |
Methods in molecular biology ; 40 |
Matèria |
|
ISBN |
0896033015 |
https://cataleg.uab.cat/record=b1736588~S1*cat
Search engines of scientific bibliography:
Scholar Google: http://scholar.google.es/advanced_scholar_search?hl=en&lr=
Scopus: http://www.scopus.com/scopus/search/form.url?display=authorLookup
Scifinder: Software disponible a la UAB
Sience Direct: http://www.sciencedirect.com/science/journals
ISI Web of Knowledge: http://www.accesowok.fecyt.es/login/
Interesting web sites:
Base de dades d’enzims BRENDA: http://www.brenda-enzymes.info/
National Center for Biotechnology Information: http://www.ncbi.nlm.nih.gov/
ExPASy (Expert Protein Analysis System) Proteomic Server: http://www.expasy.ch/