Degree | Type | Year | Semester |
---|---|---|---|
4313802 Advanced Genetics | OB | 0 | 1 |
You can check it through this link. To consult the language you will need to enter the CODE of the subject. Please note that this information is provisional until 30 November 2023.
The prerequisites for this module are those required to be admitted in the Advanced Genetics Master Program:
-B1 level of english
-Degree in Biosciences, Medicine, Pharmacy or Veterinary medicine
Basic knowledge in Molecular Genetics and laboratory skills are recomended.
This course is designed to provide the student the necessary skills to perform accurately and autonomously inside the bioscience lab, with emphasis on molecular aspects. It provides a broad grounding on several techniques commonly used in the genetic field (DNA and RNA extraction, PCR, rtPCR, cloning, cell and bacterial cultures, southern blot, etc)and an introduction to experimental design and data analysis.
- DNA and RNA extraction
- DNA extraction and purification from agarose gels
- Polymerase chain reaction (PCR)
- Qualitative reverse transcription (RT)-PCR
- Cloning of PCR products
- Transformation
- Bacterial culture
- Restriction enzyme digestion
- Miniprep
- DNA Labeling
- Transfer of PCR products into nylon membranes
- Probe-DNA hybridization
- Probe detection
- Cell culture
- In vitro toxicity assay
- Use of softwares of design and analysis (Primer, Nice, Image J, Chromas)
*Unless the requirements enforced by the health authorities demand a prioritization or reduction of these contents.
The present module is entirely practical. The student will work mostly individually, learning how to take care of its own biological samples while collaborating with other classmates. The student will go over different experimental protocols in order to solve theoretical exercises previously proposed by the teacher.
During the course, the student will also need to autonomously solve different specific questions related to the experimental design and/or the analysis of the obtained results.
At the end of the course, the student will elaborate a laboratory report with all the contents of the module.
Annotation: Within the schedule set by the centre or degree programme, 15 minutes of one class will be reserved for students to evaluate their lecturers and their courses or modules through questionnaires.
Title | Hours | ECTS | Learning Outcomes |
---|---|---|---|
Type: Directed | |||
Follow experimental protocols | 54 | 2.16 | |
Type: Supervised | |||
General laboratory work | 48 | 1.92 | |
Solve and discuss questions formulated during the experimental work | 5 | 0.2 | |
Type: Autonomous | |||
Self-study | 9 | 0.36 | |
Write a laboratory report | 12 | 0.48 |
CONTINUED EVALUATION
The final grade is composed of the following percentages:
-20% attendance, participation and laboratory skills.
-80% written document with an exhaustive description of all the laboratory exercicies developed in class. The
document will also include the answer to some specific questions proposed by the teacher during the module.
SINGLE EVALUATION
The students that required so will be able to follow a single evaluation. The single evaluation will consist on a final report similar to the continued evaluation. To pass the module it is mandatory to obtain more or equal than 5 in the final rates. This rate will be the 80% of the final module grade.
The attendance to the practical sessions is compulsory and mandatory. This part evaluation will be the same as in the continued evaluation. The obtained rate will be the 20% of the final module grade.
The sigle evaluation report will coincide with the same date in the calendar as the last test for the continued evaluation and the same recovery system as the continued evaluation will be applied.
Title | Weighting | Hours | ECTS | Learning Outcomes |
---|---|---|---|---|
Attendance, attitud and lab skills | 20% | 2 | 0.08 | 1, 5, 8, 10, 11, 12, 15, 14, 17, 9 |
Laboratory report | 80% | 20 | 0.8 | 2, 1, 3, 4, 13, 5, 7, 6, 8, 18, 10, 11, 12, 15, 14, 19, 17, 16, 9 |
Students will receive a complete list of specific bibliography and internet resources during the classes or via the Campus Virtual.
A laboratory manual with the experimental protocols to be developed during the module will also be available from the Campus Virtual.
Not required